487 / 1195
Figure 2. 3M Molecular Detection Assay
Salmonella
vs vs FDA BAM (shrimp, spinach, pet food)
1
2
Screen for
Indigenous
Salmonella
Matrix
MPN
25g + 225ml
Lactose broth,
Blend 2 min and let stand 60±5 min at room temp
Mix and adjust pH to 6.8±0.2 if necessary
24±2h h at 35°C
0.1ml sample +
10ml RV, 24±2h
at 42±0.2°C
Estimate
total Aerobic
Counts
High Microbial Load
Products
1.0ml sample + 10ml
TT, 24±2h at
43±0.2°C
Transfer typical colonies
to TSI/LIA slants
24±2h at 35°C
Confirmation of
test method
Low Microbial
Load Products
1.0ml sample +
10ml TT, 24±2h at
35±2°C
BAM
Biochemical/serological
confirmation on all
enrichments
according to
FDA-BAM
• Streak to XLD & HE - 24±2h at 35°C
• Streak to BS - incubate at 35°C for
24±2h, read and pick if applicable.
Re-incubate another 24 h
Run the 3M Molecular
Detection System
following IFU
3M
Shrimp, spinach
25g + 225ml
BPW
(prewarmed to 37C)
Homogenize 2 min
18-24h h at 37±1°C
Pet Food
375g + 3.375L
BPW
(prewarmed to 37C)
Homogenize 2 min
18-24h h at 37±1°C
NOTE - Confirmation of test method
begins with a transfer from the 3M primary
enrichment (BPW) into RV and TT.
3
27