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C. (a)
Components of the kit are intended for use as integral unit. Do not mix reagents or
1
disposables of different lot numbers.
2
C. (b)
Store VIDAS LPT kits at 2°-8°C.
3
C. (c)
Do not freeze reagents.
4
C. (d)
Bring reagents to room temperature before inserting them into the VIDAS
5
instrument.
6
C. (e)
Standard, controls and heated test portions are mixed well before using.
7
C. (f)
Include 1 positive and 1 negative control with each group of tests.
8
C. (g)
Return unused components to 2-8°C immediately after use.
9
C. (h)
See safety precautions in the VIDAS LPT package insert (refer to the following
10
sections in the package insert: Warnings and Precautions and Waste Disposal.)
11
C. (i)
Please review the polices recommend by the CDC on dealing with pathogens
12
http:/www.cdc.gov/biosafety/publications/bmb15/index.htm/
13
14
SafetyPrecautions
15
Listeria monocytogenes
is of particular concern for pregnant women, the aged
16
and the infirmed. It is recommended that these concerned groups avoid handling
17
this organism. Dispose of all reagents and other contaminated materials by
18
acceptable procedures for potentially biohazardous materials.
Some reagents in
19
the kit contain 1 g/L concentrations of sodium azide. Check local regulations
20
prior to disposal. Disposal of these reagents into sinks with copper or lead
21
plumbing should be followed immediately with large quantities of water to
22
prevent potential hazards.
This kit contains products of animal origin. Certified
23
knowledge of the origin and/or sanitary state of the animals does not totally
24
guarantee the absence of transmissible pathogenic agents. It is therefore
25
recommended that these products be treated as potentially infectious and handled
26
observing the usual safety precautions (do not ingest or inhale)
27
28
D. Preparation of Test Suspension
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D. (a)
Pre-enrichment-
Pre-enrich test portion using filter Stomacher
®
type bags to initiate
30
growth of
Listeria.
For 25 g test portions, add 225 mL of pre-warmed (18-25
o
C) LPT
31
broth to each test portion and homogenize thoroughly for 2 minutes. For cantaloupe
32
melons, soak entire melon in approximately 1 L of pre-warmed (18-25
o
C) LPT broth.
33
For 125 g test portions, add 375 mL of pre-warmed (18-25
o
C) LPT broth to each test
34
portion and homogenize thoroughly for 2 minutes.
35
D. (b)
25 g test portions/cantaloupe melons rinses
: After homogenization,incubate for 26-
36
30 hours at 30 ± 1°C.
37
125 g test portions
: After homogenization, incubate for 24-30 hours at 30 ± 1°C.
38
From the primary enrichment broth, transfer a 1 mL aliquot into 10 mL of pre-
39
warmed (18-25
o
C) LPT broth and incubate for 22-26 hours at 30± 1°C.
40
7