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Table 2013.2B

: Comparative Results for the Detection of

Listeria

spp. in Queso Fresco (25 g) by the VIDAS LPTMethod with Confirmation on

1

ALOAvs. AOAC 993.12 in a Collaborative Study (Un-inoculated Controls)

2

Statistic

MPN/

Test Portion

Laboratory

Candidate

presumptive (CP)

Candidate confirmed

(CC)

Candidate result (C)

Reference method

(R)

C vs. R

N

X

POD(CP)

N

X

POD(CC)

N

X

POD(C) N

X POD(R) dLPOD

(C,R)

dLPOD

(CP,CC)

0.00

0.00

0.11

1

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

2

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

3

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

4

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

5

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

6

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

7

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

8

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

9

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

10

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

11

a

12

0

0.00

12

0

0.00

12

0

0.00

12

7

0.58

-0.58

0.00

12

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

13

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

14

12

1

0.08

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.08

Estimate

All

156

1

0.01

156

0

0.00

156

0

0.00

156

0

0.00

0.00

0.01

LCL

0.01

0.00

0.00

0.00

-0.02

-0.02

UCL

0.04

0.02

0.02

0.02

0.02

0.04

s

r

b

0.08

0.00

0.00

0.00

LCL

0.07

0.00

0.00

0.00

UCL

0.15

0.15

0.15

0.15

s

L

c

0.00

0.00

0.00

0.00

LCL

0.00

0.00

0.00

0.00

UCL

0.03

0.15

0.15

0.15

s

R

d

0.08

0.00

0.00

0.00

UCL

0.07

0.00

0.00

0.00

LCL

0.13

0.21

0.21

0.21

P

T

e

0.4395

1.0000

1.0000

1.0000

a

Results were not used in statistical analysis due to laboratory error.

3

b

Repeatability Standard Deviation,

c

Among-Laboratory Standard Deviation,

d

Reproducibility Standard Deviation,

e

P Value =Homogeneity test of laboratory PODs

4

LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

5

6

24