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Table 2013.2A(cont’d.)

: Comparative Results for the Detection of

Listeria

spp. in Queso Fresco (25 g) by the VIDAS LPTMethod vs. AOAC 993.12 in a

1

Collaborative Study (Low Level)

2

Statistic

MPN/

Test Portion Laboratory

Candidate

presumptive (CP)

Candidate confirmed

(CC)

Candidate result (C)

Reference method

(R)

C vs. R

N

x

POD(CP)

N

x

POD(CC)

N

X

POD(C) N

X POD(R) dLPOD

(C,R)

dLPOD

(CP,CC)

0.63

0.49

0.79

1

12

6

0.50

12

6

0.50

12

6

0.50

12

5

0.42

0.08

0.00

2

12

4

0.33

12

4

0.33

12

4

0.33

12

5

0.42

-0.09

0.00

3

12

4

0.33

12

4

0.33

12

4

0.33

12

6

0.50

-0.17

0.00

4

12

7

0.58

12

7

0.58

12

7

0.58

12

7

0.58

0.00

0.00

5

12

5

0.42

12

5

0.42

12

5

0.42

12

4

0.33

0.09

0.00

6

12

6

0.50

12

6

0.50

12

6

0.50

12

6

0.50

0.00

0.00

7

12

6

0.50

12

6

0.50

12

6

0.50

12

7

0.58

-0.08

0.00

8

12

8

0.66

12

8

0.66

12

8

0.66

12

6

0.50

0.16

0.00

9

12

7

0.58

12

7

0.58

12

7

0.58

12

7

0.58

0.00

0.00

10

12

6

0.50

12

6

0.50

12

6

0.50

12

6

0.50

0.00

0.00

11

a

12

6

0.50

12

6

0.50

12

6

0.50

12

8

0.66

-0.16

0.00

12

12

7

0.58

12

7

0.58

12

7

0.58

12

6

0.50

0.08

0.00

13

12

7

0.58

12

7

0.58

12

7

0.58

12

5

0.42

0.16

0.00

14

12

7

0.58

12

5

0.42

12

5

0.42

12

6

0.50

-0.08

0.16

Estimate

All

156 80

0.51

156 78

0.50

156 78

0.50

156 76

0.49

0.01

0.01

LCL

0.43

0.42

0.42

0.41

-0.10

-0.10

UCL

0.59

0.58

0.58

0.57

0.13

0.13

s

r

b

0.51

0.51

0.51

0.52

LCL

0.46

0.46

0.46

0.46

UCL

0.52

0.52

0.52

0.52

s

L

c

0.00

0.00

0.00

0.00

LCL

0.00

0.00

0.00

0.00

UCL

0.13

0.14

0.14

0.10

s

R

d

0.51

0.51

0.51

0.52

UCL

0.46

0.46

0.46

0.47

LCL

0.52

0.52

0.52

0.52

P

T

e

0.9210

0.9161

0.9161

0.9937

a

Results were not used in statistical analysis due to laboratory error.

3

b

Repeatability Standard Deviation,

c

Among-Laboratory Standard Deviation,

d

Reproducibility Standard Deviation,

e

P Value=Homogeneity test of laboratory PODs

4

LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

5

6

22