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Table 2013.2B (cont’d)

: Comparative Results for the Detection of

Listeria

spp. inQueso Fresco (25 g) by the VIDAS LPTMethod with Confirmation on

1

ALOAvs. AOAC 993.12 in a Collaborative Study (High Level)

2

Statistic

MPN/

Test Portion Laboratory

Candidate

presumptive (CP)

Candidate confirmed

(CC)

Candidate result (C)

Reference method

(R)

C vs. R

N

X

POD(CP)

N

X

POD(CC)

N

X

POD(C) N

X POD(R) dLPOD

(C,R)

dLPOD

(CP,CC)

5.48

3.60

8.36

1

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

2

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

3

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

4

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

5

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

6

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

7

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

8

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

9

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

10

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

11

a

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

12

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

13

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

14

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

Estimate

All

156 156

1.00

156 156

1.00

156 156

1.00

156 156

1.00

0.00

0.00

LCL

0.98

0.98

0.98

0.98

-0.02

-0.02

UCL

1.00

1.00

1.00

1.00

0.02

0.02

s

r

b

0.00

0.00

0.00

0.00

LCL

0.00

0.00

0.00

0.00

UCL

0.15

0.15

0.15

0.15

s

L

c

0.00

0.00

0.00

0.00

LCL

0.00

0.00

0.00

0.00

UCL

0.15

0.15

0.15

0.15

s

R

d

0.00

0.00

0.00

0.00

UCL

0.00

0.00

0.00

0.00

LCL

0.21

0.21

0.21

0.21

P

T

e

1.0000

1.0000

1.0000

1.0000

a

Results were not used in statistical analysis due to laboratory error.

3

c

Repeatability Standard Deviation,

d

Among-Laboratory Standard Deviation,

e

Reproducibility Standard Deviation,

f

P Value =Homogeneity test of laboratory PODs

4

LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

5

6

26