606 / 1195
a)
Kit Name.-
VIDAS®
L. monocytogenes
Xpress (LPT)
1
b)
Catalog Number.-RF30126
2
c)
Test Kit Components
3
a.
60 LPT Strips.
4
b.
60 LPT SPR®s
.-Interior of SPR®s coated with proteins specific for
Listeria
5
receptors.
6
c.
LPT Standard (1 x 6 mL).-
Ready-to-use. Purified and inactivated
L.
7
monocytogenes
antigen + preservative + protein stabilizer. The confidence
8
interval is indicated on the MLE card after the following mention: "Standard
9
(S1) RFVRange".
10
d.
LPT Positive Control (1 x 6 mL)
.-Purified and inactivated
L. monocytogenes
11
antigen + preservative + protein stabilizer. The confidence interval is
12
indicated on the MLE card after the following mention: "Control C1 (+)
13
TestValueRange".
14
e.
Negative Control (1 x 6 mL)
.-TRIS buffered saline (TBS) (150 mmol/L) -
15
Tween pH 7.6 + preservative. The maximum acceptable value for the test is
16
indicated on the MLE card after the following mention: "Control C2 (-)
17
TestValueRange".
18
f.
1 MLE Card (Master Lot Entry)
.-Specifications for the factory master
19
calibration data required to calibrate the test: to read the MLE data, please
20
refer to the Operator’s Manual.
21
22
Additional Supplies and Reagents
23
a)
LPT Broth
225ml (bioMerieux 410848)
24
b)
LPT Broth 3l (réf. bioMérieux 410849)
25
c)
LPT Broth 100 ml (réf. bioMérieux 410846)
26
d)
LPT Broth 10 ml (réf. bioMérieux 410845)
27
e)
ALOA® agar - (AES ref. AEB520079)
28
29
Apparatus
30
a)
VIDAS® instrument
.
31
b)
Disposable pipettes
- to dispense 500 μL.
32
c)
VIDAS® Heat and Go
- (bioMérieux ref. 93554 or 93555 or 93556) or a water-bath
33
(95-100°C) or an equivalent system.
34
d)
Stomacher-type mixer
.Smasher™ Blender/Homogenizer
35
e)
Stomacher-type bag with filter
.
36
37
Safety Precautions
38
Listeria monocytogenes
is of particular concern for pregnant women, the aged and
39
the infirmed. It is recommended that these concerned groups avoid handling this
40
organism. Dispose of all reagents and other contaminated materials by acceptable
41
procedures for potentially biohazardous materials.
Some reagents in the kit contain
42
1 g/L concentrations of sodium azide. Check local regulations prior to disposal.
43
Disposal of these reagents into sinks with copper or lead plumbing should be
44
followed immediately with large quantities of water to prevent potential hazards.
45
This kit contains products of animal origin. Certified knowledge of the origin and/or
46
sanitary state of the animals does not totally guarantee the absence of transmissible
47
pathogenic agents. It is therefore recommended that these products be treated as
48
45