861 / 1195
VIDAS LMX & LPT Collaborative Study
April 19, 2013
OMA-2013-Apr-XXX
Appendix 9.4
1
Study Materials
2
3
Materials provided by collaborator
4
5
1.
Balance. (
Weight range and tolerance of the balance should be 0.1 g as is recommended in
6
the BAM)
7
2.
Pipette with disposable tips.
– Calibrated to dispense 0.5 mL.
8
3.
Vortex mixer.
9
4.
Inoculating loops.
10
5.
Stomacher lab blender 400, or equivalent
– Available from Seward Medical, London, UK.
11
6.
Stomacher bags with filters. –
For test portion homogenization and enrichment.
12
7.
Incubators.
– Capable of maintaining 30 and 37 ± 1°C
13
8.
VIDAS® System
. – Available from bioMérieux, Inc., 595 Anglum Rd., Hazelwood, MO
14
63042-2320, USA.
15
9.
Heat and Go.
– Available from bioMérieux, Inc., 595 Anglum Rd., Hazelwood, MO 63042-
16
2320, USA.
17
18
Materials and reagents provided by the study sponsor
19
20
VIDAS LMX kits
21
LMX broth
22
LMX supplement
23
VIDAS LPT kits
24
LPT broth
25
26
Enrichment
27
Selective enrichment medium (AOAC)
28
29
Selective agar
30
ALOA Agar
31
Oxford Agar
32
33
Confirmation
34
Blood agar
35
Catalase
36
Motility supplies
37
Tryptic Soy Agar with yeast extract
38
VITEK2 GP or API Listeria
39
DRAFT DOCUMENT
24