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VIDAS LMX & LPT Collaborative Study
April 19, 2013
OMA-2013-Apr-XXX
Appendix 9.5a – Flow Diagram for Soft Mexican Cheese, 25g LMX
1
2
25g + 225ml selective
enrichment medium
(prewarm to 45C)
Blend 1-3 min
Incubate 48 h at 30C
AOAC
VIDAS
Dairy
Confirm at least one colony per
sample according to AOAC
993.12 (VITEK2GP or API
Listeria)
Adjust pH to 7.3, if necessary
At 48 h, streak to OX
Incubate 48 h at 37C
Streak typical colonies
to TSAYE
24-48 h at 37C
25g + 225ml LMX broth
(prewarm to 18-25C) +
supplement
26-30h at 37±1C
Confirm at least one colony per
sample according to AOAC
993.12 (VITEK2GP or API
Listeria)
Streak to OX
Incubate 24-48 h
at 37C
Streak typical colonies
to TSAYE
24-48 h at 35C
Confirm at least one colony per
sample according to AOAC
993.12 (VITEK2GP or API
Listeria)
Streak to ALOA
Incubate 24-48 h
at 35-37C
Streak typical colonies
to TSAYE
24-48 h at 35C
Option 1:
Transfer 0.25 ml into
LMX
strip,
heat 5±1 min on the Heat & Go, cool for
10 min, run VIDAS
Option 2:
Boil 1-2 mL 5±1 min at 95-100C,
cool for 10 min, 0.25 mL into
LMX
strip,
run VIDAS
3
DRAFT DOCUMENT
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