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1
Liver pate
2
For liver pate inoculated with
L.monocytogenes
(unpaired enrichments), for the LMX method there were 11 confirmed positives at the
3
low level and 15 confirmed positives at the high level. For the MFHPB30 reference method there were 10 confirmed positives at the
4
low level and 12 confirmed positives at the high level. All uninoculated samples were negative with both methods. There were no
5
false positives for the VIDAS method for uninoculated samples. There was no significant difference between the LMX method and
6
the reference method using unpaired Chi-square test. (Table 13)
7
8
Hot dogs
9
For hot dogs inoculated with
L.monocytogenes
(unpaired enrichments), for the LMX method there were 10 confirmed positives at the
10
low level and 20 confirmed positives at the high level. For the MFHPB30 reference method there were 8 confirmed positives at the
11
low level and 13 confirmed positives at the high level. All uninoculated samples were negative with both methods. There were no
12
false positives for the VIDAS method for uninoculated samples. Confirmation using OAA or ChromID Lmono media showed exact
13
agreement with traditional media. There was a significant difference between the LMX method and the reference method at the high
14
level inoculation (Chi-square= 8.27) for hot dogs favoring the VIDAS LMX. (Table 13)
15
16
Raw fermented sausage
17
For sausage inoculated with
L.monocytogenes
(unpaired enrichments) for the LMX method there were 9 confirmed positives at the
18
low level and 11 presumptive positives versus 12 confirmed positives at the high level, resulting in one false negative result for LMX.
19
For the MFHPB30 reference method there were 10 confirmed positives at both the low level and high level. All uninoculated samples
20
were negative with both methods. There were no false positives for the VIDAS method for uninoculated samples. Confirmation using
21
OAA or ChromID Lmono media showed exact agreement with traditional media. There was no significant difference between the
22
LMX method and the reference method using unpaired Chi-square test. (Table 13)
23
24
Deli turkey
25
For deli turkey inoculated with
L.monocytogenes
(unpaired enrichments), for the LMX method there were 15 confirmed positives at
26
the low level and 15 confirmed positives at the high level. For the MFHPB30 reference method there were 15 confirmed positives at
27
the low level and 17 confirmed positives at the high level. All uninoculated samples were negative with both methods. There were no
28
false positives for the VIDAS method for uninoculated samples. Confirmation using OAA or ChromID Lmono media showed exact
29
agreement with traditional media. There was no significant difference between the LMX method and the reference method using
30
unpaired Chi-square test. (Table 13)
31
32
33
Deli ham
34
For deli ham inoculated with
L.monocytogenes
(unpaired enrichments), for the LMX method there were 10 confirmed positives at the
35
low level and 16 confirmed positives at the high level. For the MFHPB30 reference method there were 13 confirmed positives at the
36
low level and 18 confirmed positives at the high level. All uninoculated samples were negative with both methods. There were no
37
PTM Certification Report