944-945 / 1195
27
false positives for the VIDAS method for uninoculated samples. Confirmation using OAA or ChromID Lmono media showed exact
1
agreement with traditional media. There was no significant difference between the LMX method and the reference method using
2
unpaired Chi-square test. (Table 13)
3
4
Independent Study Results:
5
6
Vanilla Ice cream
7
For ice cream inoculated with
L.monocytogenes
(unpaired enrichments) there were 8 confirmed positives for the LMX method and 12
8
confirmed positives for the USDA reference method. All uninoculated samples were negative with both methods. There were no false
9
positives for the VIDAS method for uninoculated samples. Confirmation using OAA or ChromID Lmono media showed exact
10
agreement with traditional media. There was no significant difference between the LMX method and the reference method using
11
unpaired Chi-square test. (Table 12)
12
13
Cooked shrimp
14
For cooked shrimp inoculated with
L.monocytogenes
(unpaired enrichments) there were 7 confirmed positives for the LMX method
15
and 6 confirmed positives for the USDA reference method. All uninoculated samples were negative with both methods. There were
16
no false positives for the VIDAS method for uninoculated samples. Confirmation using OAA or ChromID Lmono media showed
17
exact agreement with traditional media. There was no significant difference between the LMX method and the reference method using
18
unpaired Chi-square test. (Table 12)
19
20
Deli ham
21
For deli ham inoculated with
L.monocytogenes
(unpaired enrichments) there were 16 confirmed positives for the LMX method and 14
22
confirmed positives for the USDA reference method. All uninoculated samples were negative with both methods. There were no false
23
positives for the VIDAS method for uninoculated samples. Confirmation using OAA or ChromID Lmono media showed exact
24
agreement with traditional media. There was no significant difference between the LMX method and the reference method using
25
unpaired Chi-square test. (Table 12)
26
27
Independent lab discussion and observations
28
Results obtained from samples analyzed by the VIDAS
®
LMX method were comparable to those analyzed by the reference methods.
29
The Mantel-Haenszel chi-square analysis for unpaired samples was performed between the VIDAS
®
LMX method and the reference
30
methods. For Sliced Deli Ham the
χ
2
was 0.52; for Vanilla Ice Cream the
χ
2
was 1.56; and for Cooked Shrimp the
χ
2
was 0.11
31
indicating there was no significance difference between the VIDAS
®
LMX method and all three reference methods at a 95%
32
confidence level. The ChromID agars, OAA and Lmono agar had a 100% correlation with the selective isolation agars of the
33
reference methods.
34
35
PTM Certification Report