964 / 1195
Eurofins IPL Nord SAS – 1 rue du professeur Calmette – 59046 LILLE cedex
9 / 15
VIDAS LMX – Summary 2012 v01
3.2 Control of experimental parameters
3.2.1 Contamination levels obtained after artificial contamination
The following table shows the contamination rates obtained and estimated precisions :
Level
Samples
Targeted theoretical
rate (CFU/25ml)
Real rate
(CFU/25ml)
Estimated lower
contamination limit
per 25 ml sample
Estimated upper
contamination limit
per 25 ml sample
Level 0 (L0)
5-6-9-10-13-14-19-20
27-28-35-36-41-42-43-44
0
0
/
/
Low level (L1)
3-4-11-12-17-18-23-24
29-30-31-32-39-40-45-46
3
3,26
0,7
9,5
High level (L2)
1-2-7-8-15-16-21-22
25-26-33-34-37-38-47-48
30
22,2
14
33
3.2.2 Problems of temperature recorded during transport, temperature on reception and
reception times
a)
Analysis of temperature monitoring curves during transport
Temperatures registered by thermo button during shipment were stables and comprised between 0°C and 8° C for
all laboratories.
b)
Temperatures on reception and reception times
The temperatures obtained are recorded in the following table :
Laboratory
Reception Temperatures (°C)
Comments
communicated by the laboratory
indicated by the thermo button
A
3.1
1.2
B
4.5
2.5
C
3.3
1.5
D
4.2
4.0
E
2.7
3.0
F
Not communicated
2.5
Withdrawal
G
Not communicated
1.5
H
2.6
3.0
I
6.0
3.5
J
4.1
4.0
Reception at D1, assays at D2
K
6.1
0.1
L
4.8
-0.4
M
1.4
2.1
N
3.9
3.5
O
7.0
/
Reception at D2
P
6.0
4.1
Reception at D2
Among the16 laboratories, 14 laboratories received samples the day after the sending.
3.2.3 Conclusion
Continuation in the conditions of transport of samples, the results are exploitable for 12 laboratories (exclusion from
laboratories C, F, J, I, L and P).
Further to the sending of samples in 16 laboratories, the results are exploitable for 12 laboratories (exclusion from
laboratories J, O and P continuation in the conditions of reception of samples at D2 and withdrawal of the
laboratory F).