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Eurofins IPL Nord SAS – 1 rue du professeur Calmette – 59046 LILLE cedex
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VIDAS LMX – Summary 2012 v01
In the case of positive samples, it is necessary to add the time necessary for the confirmations.
The interest of the alternative method is the possibility of screening the negative samples and of reducing the
number of confirmations.
Furthermore, time of manipulations are widely reduced with regard to the reference method, for which 5
confirmations of suspect colonies by plated agar are necessary for negative samples (negative confirmation).
9. Time to result
Step
Time required (Day)
VIDAS LMX method
Time required (Day)
EN ISO 11290-1 standard
Realization of pre-enrichment
D0
D0
Inoculation of Fraser 1
/
D1
Inoculation of LX broth
D1 (only specific protocol)
/
Streaking on selective media
/
D1 & D3
Realization of VIDAS LMX test
D1
/
Reading the plates
/
D4
Obtaining negative results
(if no characteristic colony
)
D1
D4 to D5
Confirmation tests
/
D2 to D5
Obtaining negative results
(after negative confirmation if necessary)
D2
D5 to D11
Obtaining positive results
(confirmation of typical colonies)
D2 to D8
D8 to D11
-
Confirmation by reference method tests (CAMP-test,
haemolysis,..), including purification
-
Confirmation by reference method tests (CAMP-test,
haemolysis, sugars (identification galery)
-
Confirmation by plating on chromogenic medium
D4 to D8
D4
D2
D4 to D7
/
/
10. Type of qualification of the
operator
Level identical to that for the reference method
11. Steps common to the
reference method
Confirmations (reference method tests including purification)
12. Traceability of the analysis
results
1 MLE card (Master Lot Entry) : specifications sheet containing the factory
master calibration data required to calibrate the test.
A result sheet is printed with the reagents lot numbers, time, test result, and
sample identification and interpretation.
13.
Maintenance
by
the
laboratory
The VIDAS user’s guide explains some problems.
BioMerieux offers a phone customar technical support for the possible
problems. Different maintenance contracts are possible.
Calibration, using the standard provided in the kit, must be performed each
time a new lot of reagents is opened, after the master lot data have been
entered. Calibration should then be performed every 28 days. This operation
provides instrument specific calibration curves and compensates for possible
minor variations in assay signal throughout the shelf-life of the kit.