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Eurofins IPL Nord SAS – 1 rue du professeur Calmette – 59046 LILLE cedex

VIDAS LMX – Summary 2012 v01

EN ISO STANDARD 11290-1/A1: 2005 (#)

Incubation 48 h

at 37°C ±1°C

YES

YES

YES

NO

NO

Incubation 24 h at 37°C ±1°C

Incubation 24 h

at 37°C ±1°C

Test portion (x g or x ml)

Dilution 1/10 in Fraser ½ broth

Plating on selective

Agar Listeria OAA & Oxford

Transfer 0,1 ml of Fraser ½

enrichment in 10 ml Fraser 1

Plating on selective

Agar Listeria OAA & Oxford

Presence of typical

colonies of

Listeria

Re-incubate 24 h at 37°C ±1°C

Presence of typical

colonies of

Listeria

Confirmation

L. monocytogenes

Response :

Absence of

L. monocytogenes

in x g

Response :

Presence of

L. monocytogenes

in x g

Incubation 24 h at 30°C ±1°C

NO