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VIDAS LMX – Summary 2012 v01
VIDAS LMX ALTERNATIVE METHOD
Diagram of procedure of specific protocol for the analysis of raw milk cheeses
YES
YES
NO
Enrichment
Test portion 25 g
in 450 ml LMX broth (1/20) preheated at 37°C in a b ag filter
Incubation 20 h to 24 h at 37°C ±1°C
After homogenization of second enrichment :
-
transfer 1-2 ml in a tube and heat at 95-100°C for 5 ± 1 minutes, cool the
tube, mix and transfer 250 µl into the sample well on the VIDAS
®
Strip
-
or if the VIDAS
®
Heat & Go is used, transfer 250 µl of enrichment broth into
the sample well on the Strip. Heat for 5 ± 1 minutes. Remove the strip and
leave to cool for 10 minutes
Perform the VIDAS
®
test
Positive
VIDAS LMX test
Response :
Absence of
L. monocytogenes
in x g
Confirmations
Response :
Presence of
L. monocytogenes
in x g
NO
After homogenization of enrichment,
transfer 3 ml in 6 ml LX broth
Incubation 6 h to 8 h at 37°C ±1°C