984 / 1195
Eurofins IPL Nord SAS – 1 rue du professeur Calmette – 59046 LILLE cedex
VIDAS LMX – Summary 2012 v01
VIDAS LMX ALTERNATIVE METHOD
Diagram of procedure of General protocol
YES
YES
NO
Enrichment
Test portion (x g or x ml)
in 9x ml LMX broth supplemented with selective agents
preheated at 37°C in a bag filter
Incubation 26 h to 30 h at 37°C ±1°C
After homogenization of enrichment :
-
transfer 1-2 ml in a tube, seal and heat at 95-100°C for 5 ± 1 minutes, cool the
tube, mix and transfer 250 µl into the sample well on the VIDAS
®
Strip
-
or if the VIDAS
®
Heat & Go is used (except ovoproducts and poultry products),
transfer 250 µl of enrichment broth into the sample well on the Strip. Heat for 5
± 1 minutes. Remove the strip and leave to cool for 10 minutes
Perform the VIDAS
®
test
Positive
VIDAS LMX test
Response :
Absence of
L. monocytogenes
in x g
Confirmations
Response :
Presence of
L. monocytogenes
in x g
NO