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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
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compounds after adjusting their values to folic acid.
Spike Recovery Evaluation in SPIFAN samples at different spike levels:
Two beverages prepared from the respective powdered samples and three RTF samples were analyzed
for all the targeted folate compounds with and without spike with respective folate compounds. The
samples were spiked with the folate compounds.
Table 28: % Spike Recovery of folate compounds in SPIFAN samples spiked with folate
compounds at three different spike levels. The results evaluated for total folate (sum of evaluated
folate compounds after adjustment to folic acid).
Sample
#
Sample
Lot #/ID
Matrix
Analyzed
as
% Spike
Recovery
Parameters
Spike 1 T=
492; I = 75
mcg/100g*
Spike 2 T
= 126; I =
19
mcg/100g*
Spike 3 T
= 63; I = 10
mcg/100g*
All
Levels*
1
Child
Formual
Powder
004011RF00
Powder
Reconst.
Beverage
Av % Recov
105.2
103.2
103.3
103.9
SD
1.0
1.2
3.5
2.1
% RSD
0.9
1.2
3.4
2.1
2
Infant
Formula
Powder
00402RF00
Powder
Reconst.
Beverage
Av % Recov
100.7
97.0
98.0
98.6
SD
2.6
1.8
1.5
2.4
% RSD
2.6
1.8
1.5
2.4
3
Adult
Nutritional
RTF High
Protein
00415RF00
Beverage
As is
Av % Recov
93.5
90.5
93.2
92.4
SD
0.0
1.4
0.4
1.6
% RSD
0.0
1.6
0.4
1.7
4
Adult
Nutritional
RTF High Fat
00407RF00
Beverage
As is
Av % Recov
91.3
87.6
98.7
92.6
SD
1.7
1.4
7.1
6.2
% RSD
1.9
1.6
7.2
6.7
5
Infant
Formula
Powder
Milk Based,
SPIFAN
Blank Milk
Form
00394RF00
Beverage
As is
Av % Recov
101.6
101.2
100.7
101.2
SD
1.0
3.8
2.5
2.369
% RSD
0.9
3.7
2.5
2.3
* Samples were spiked with each targeted folate compound. Total Folic calcluted as sum of the folate compnds
after adjusting their values to folic acid. Spike levels are for Total Folate (T) and individual folate compounds (I)
Each provided value is an average of three independent replicates. Spike recovery of the different spike
levels was evaluated on different days.
The liquid beverage samples and the RTF liquid samples were analyzed for folate compounds and
expressed as total folate after adjusting to folic acid in and % spike recovery calculated in sample at
different spike levels (I, II and II). Results are presented in the above Table. All the 5 samples received
from SPIFAN were analyzed in replicates
at different spike levels were analyzed on different days. All
replicates of the same spike level samples were analyzed on the same day. The samples were spiked
with each targeted folate compound (at equal level of each of seven targeted compound) and sum of all
folate compounds as folic acid provided folic acid spike level for the sample. Spike levels were
calculated as mcg/100g (reconstituted beverage/RTF).
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE