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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
34
Table 25 : Reconstitution of the powder samples (for method repeatability/precision evaluation)
before the analysis
Sample
Matrix
Lot #/ID
REC'D
AS
Reconstitution*
LCS
Breakfast Cereal
Sample
Weight
In to
H
2
O
(mL)
1
SRM 1849A
CLC10-b
POWDER 25.0926
200
2
Adult Nutritional Powder
Milk Protein Based
11750017V3
POWDER 25.0158
200
3
Infant Formula Powder
Partially Hydrolyzed Milk
Based
1172572116
POWDER 25.0324
200
4
Infant Formula Powder
Partially Hydrolyzed Soy
Based
117257651Z
POWDER 25.0356
200
5
Adult Nutritional Powder
Low Fat
00394RF00
POWDER 25.0142
200
6
Child Formual Powder
00412RF00
POWDER 25.0264
200
7
Infant Elemental Powder
00403RF00
POWDER 25.0108
200
8
Infant Formula Powder
Milk Based
D04HTCVV
POWDER 25.017
200
9
Infant Formula Powder
Soy Based
E29JVLV
POWDER 25.1178
200
10
Infant Formula RTF Milk
Based
SPIFAN
CONTROL MILK
FORM
LIQUID
-
-
11
Adult Nutritional RTF High
Protein
00414RF00
LIQUID
-
-
12
Adult Nutritional RTF High
Fat
00406RF00
LIQUID
-
-
* All the samples received as powder were recostituted and and the
reconstituted beverage was analyzed for folates.
Averages and Precision of within day Replicates analysis of SPIFAN sample tested for separate
three days:
Nine beverages prepared from the respective powdered samples and three RTF samples were analyzed
for all the targeted folate compounds. The folate compounds in each powder sample was calculated on
as is basis (powder basis). Similarly the folate compounds in the RTF samples were also calculated as
is basis (liquid basis). Each folate compound analyzed was converted into folic acid and sum of that
provided total folate contents in the sample (expressed as folic acid) and reported as mcg/g (ppm). Each
sample was analyzed in three replicates for three separate days. Results for the averages and
repeatability/precision (%RSD
r
) for each for every day of the analysis are calculated. The results are
presented in the following Table.
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE