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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
30
Table 20: 5-methyl-THF analysis in NIST SRM 1849a (Milk based Infant Formula) over a period
of about 3 months
5-CH3-THF
Results
08/28/12 09/11/12 09/21/12 09/26/12 09/28/12 10/02/12 10/12/12 10/19/12
mcg/g
Mean
0.146
0.125
0.153
0.173
0.177
0.185
0.159
0.183
%RSD
3.3%
0.6%
12.5%
2.7%
1.8%
8.8%
2.3%
2.6%
N =
3
3
3
3
3
2
3
3
5-CH3-THF
Results
10/26/12 11/07/12 11/13/12 11/17/12 11/26/12 11/29/12 Grand
Grand
SD
mcg/g
Mean
0.195
0.141
0.173
0.170
0.165
0.181
0.166
0.020
%RSD
2.6%
5.3%
2.3%
3.9%
1.8%
2.4%
12.1
N =
3
3
3
3
3
3
41
41
Ref Value*
0.084 + 0.031
NIST Reference Value, it's not a certified value based on NIST LC-MS in house value
5-methyl-THFresults obtained for the BCR 485 (dried vegetable powder) by the method over a period
of about 3 months are presented in Table 27. The results obtained were quite consistent over period of
analysis. 5-methyl-THF in BCR 485 was estimated by the method close to the reported upper range.
Interday as well as intraday precision of the method over a period of about 3 months was found to be
satisfactory.
5-formyl-THFresults obtained for the BCR 485 (dried vegetable powder) by the method over a period
of about 3 months are presented in Table 28. The results obtained were quite consistent over period of
analysis. The BCR has not reported any value for 5-formyl-THF in this matrix. Interday as well as
intraday precision of the method over a period of about 3 months was found to be satisfactory.
Table 21: 5-methyl-THF analysis in BCR 485 (dried vegetable powder) over a period of about 3
months
5-CH3-THF Results 09/11/12 09/14/12 09/18/12 09/21/12 10/10/12 10/12/12 10/19/12
mcg/g
Mean
2.291
2.453
2.179
2.530
2.900
2.881
2.881
%RSD
3.4%
0.9%
1.5%
3.2%
1.7%
-
2.1%
N =
3
3
3
3
3
1
3
5-CH3-THF Results 10/26/12 11/07/12 11/13/12 12/27/12 Grand
Grand
SD
mcg/g
Mean
2.949
2.613
2.465
2.708
2.615
0.249
%RSD
0.4%
2.2%
0.5%
3.2%
9.5
N =
3
3
3
6
35
35
Indicative Value*
2.14 + 0.42
*BCR Indicative Value, is not a certified value obtained by BCR analysis using a LC fluorescence
method and hog kidney conjugase based extraction and SPE clean-up.
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE