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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
35
Table 26: Results of the SPIFAN samples for total folate calculated as sum of the detected folate
compounds and expressed as mcg/100 g of the reconstitute beverage liquid sample or RTF liquid
sample. Averages and precision (as % RSD) of replicates analyzed on the same day for separate 3
days (total n=9).
Sample
Matrix
Lot #/ID
REC'D
AS
Analyzed
AS
Av.
mcg/100g
RTF*
Stdev %RSD
n
Independent
Analysis
Days
LCS**
Breakfast Cereal
Sample
Silliker
LCS
Powder
Powder
65.2
2.9
4.5
9
3
1
SRM 1849A
CLC10-b
Powder
RTF
Liquid
31.8
2.5
7.9
9
3
2
Adult Nutritional Powder
Milk Protein Based
11750017V3
Powder
RTF
Liquid
35.6
4.5
12.7
9
3
3
Infant Formula Powder
Partially Hydrolyzed Milk
Based
1172572116
Powder
RTF
Liquid
20.0
2.5
12.5
9
3
4
Infant Formula Powder
Partially Hydrolyzed Soy
Based
117257651Z
Powder
RTF
Liquid
25.3
2.2
8.9
9
3
5
Adult Nutritional Powder
Low Fat
00394RF00
Powder
RTF
Liquid
42.2
4.1
9.7
9
3
6
Child Formual Powder
00412RF00
Powder
RTF
Liquid
24.6
1.8
7.5
9
3
7
Infant Elemental Powder
00403RF00
Powder
RTF
Liquid
32.6
3.0
9.3
9
3
8
Infant Formula Powder
Milk Based
D04HTCVV
Powder
RTF
Liquid
21.5
1.5
7.0
9
3
9
Infant Formula Powder
Soy Based
E29JVLV
Powder
RTF
Liquid
22.5
2.4
10.5
9
3
10
Infant Formula RTF Milk
Based
SPIFAN
CONTROL
MILK FORM
LIQUID
RTF
Liquid
19.5
2.1
10.5
9
3
11
Adult Nutritional RTF High
Protein
00414RF00
LIQUID
RTF
Liquid
56.0
5.5
9.9
9
3
12
Adult Nutritional RTF High
Fat
00406RF00
LIQUID
RTF
Liquid
66.2
6.5
9.8
9
3
* Total Folate Results are calculated based on sum of all the folate compounds in the samples
after the conversion of each compound as folic acid. Results are provided as mcg/g per 100 g of RTF
**Total Folate Values for Cereal sample is per 100g of the solid sample
The results of total folate as folic acid (sum of the detected folate compounds after calculation as folic
acid) in the SPIFAN samples are presented in the above Table. The samples were analyzed in triplicates
for three separate days. Averages and repeatability value/precision (%RSDr) for within day analysis are
presented. The repeatability/precision (% RSDr) with one exception were less than 7% provided in the
SMPR for all the SPIFAN samples for all three days of the analysis.
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE