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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
32
Table 24: Total folate as folic analysis in BCR 485 (dried vegetable powder) and other reference
materials
Sample
Matrix
Expected
Total Folates
(mcg/g)
Average
Total
Folates
(mcg/g)
Stdev
%RSD
n
days
NIST 1849A
infant formulas
2.293 +/-
0.062
2.826
0.2
6.9
23
8
NIST 3233
cereal
15.1 +/- 1.2
14.642
1.1
7.3
24
8
BCR 485
mixed vegetables
3.15 +/- 0.28
3.05
0.3
10.3
25
9
LCS Material
cereal
-
0.678
0.1
7.4
36
12
Analysis of the SPIFAN Matrices:
Samples:
A kit of all of the SPIFAN samples was received from SPIFAN in March of 2014. Sample Details are
provided on page 1 and reproduced here for the convenience:
Provided to Silliker by SPIFAN on 3/26/14
1.)
Child Formula Powder
Lot # 00411RF00
2.)
Infant Elemental Powder
Lot # 00402RF00
3.)
Adult Nutritional RTF, High Protein
Lot # 00415RF00
4.)
Adult Nutritional RTF, High Fat
Lot # 00407RF00
5.)
Infant Formula RTF, Milk Based
SPIFAN Blank Milk Form
The above blank samples are used for the evaluation of spike recovery of folates by the method
6.)
SRM 1849A
Lot # CLC10-b
7.)
Adult Nutritional Powder Milk Protein Based
Lot # 11750017V3
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE