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immersion thermometer, digital thermocouple thermometer, not a total immersion thermometer) placed in
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the designated location, verify that the 3M Molecular Detection Heat Block Insert is at 100 ±1°C.
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G. P
REPARATION OF THE
3MM
OLECULAR
D
ETECTION
I
NSTRUMENT
3
1.
Launch the 3M™ Molecular Detection Software and log in.
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2.
Turn on the 3M Molecular Detection Instrument.
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3.
Create or edit a run with data for each sample. Refer to the 3M Molecular Detection
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System User Manual for details.
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NOTE:
The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C before
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inserting the 3M Molecular Detection Speed Loader Tray with reaction tubes. This heating step takes
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approximately 20 minutes and is indicated by an ORANGE light on the instrument’s status bar. When the
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instrument is ready to start a run, the status bar will turn GREEN.
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H. L
YSIS
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1.
Allow the lysis solution (LS) tubes to warm up by setting the rack at room temperature (20-
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25 °C) overnight (16-18 hours). Alternatives to equilibrate the LS tubes to room
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temperature are to set the LS tubes on the laboratory bench for at least 2 hours, incubate the
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LS tubes in a 37 ±1°C incubator for 1 hour or place them in a dry double block heater for
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30 seconds at 100°C.
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2.
Invert the capped tubes to mix. Proceed to next step within 4 hrs.
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3.
Remove the enrichment broth from the incubator.
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4.
One LS tube is required for each sample and the Negative Control (NC) (sterile enrichment
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medium) sample.
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4.1
LS tube strips can be cut to desired LS tube number. Select the number of individual LS
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tubes or 8-tube strips needed. Place the LS tubes in an empty rack.
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4.2
To avoid cross-contamination, decap one LS tubes strip at a time and use a new pipette
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tip for each transfer step.
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4.3
Transfer enriched sample to LS tubes as described below:
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Transfer each enriched sample into individual LS tube
first
. Transfer the NC
last
.
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4.4
Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one LS tube strip -
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one strip at a time.
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4.5
Discard the LS tube cap – if lysate will be retained for retest, place the caps into a clean
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container for re-application after lysis
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4.6
Transfer 20 µL of sample into a LS tube.
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5.
Repeat step 4.2 until each individual sample has been added to a corresponding LS tube in
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the strip as illustrated below.
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AOAC Research Institute
Expert R view Panel Use Only
OMAMAN-29 A/ Collaboartive Study Manuscript
OMA ERP June 2016
ERP Use Only