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The 3M™ Molecular Detection instrument is intended for use with samples that have
1
undergone heat treatment during the assay lysis step, which is designed to destroy
2
organisms present in the sample. Samples that have not been properly heat treated
3
during the assay lysis step may be considered a potential biohazard and should NOT
4
be inserted into the 3M MDS instrument.
5
The user should read, understand and follow all safety information in the instructions
6
for the 3M MDS and the 3M MDA 2 –
Listeria
. Retain the safety instructions for
7
future reference.
8
To reduce the risks associated with exposure to chemicals and biohazards:
9
Performpathogen testing in a properly equipped laboratory under the control of
10
trained personnel.Always follow standard laboratory safety practices, including
11
wearing appropriate protective apparel and eye protection while handling reagents
12
and contaminated samples.Avoid contact with the contents of the enrichment media
13
and reagent tubes after amplification. Dispose of enriched samples according to
14
current industry standards.
15
Listeria monocytogenes
is of particular concern for pregnant women, the aged and the
16
infirmed. It is recommended that these concerned groups avoid handling this
17
organism.After use, the enrichment medium and the 3M MDA 2 -
Listeria
tubes can
18
potentially contain pathogenic materials. Periodically decontaminate laboratory
19
benches and equipment (pipettes, cap/decap tools, etc.) with a 1- 5% (v:v in water)
20
household bleach solution or DNA removal solution. When testing is complete,
21
follow current industry standards for the disposal of contaminated waste. Consult the
22
Safety Data Sheet for additional information and local regulations for disposal.
23
To reduce the risks associated with environmental contamination:Follow current
24
industry standards for disposal of contaminated waste.
25
D.
Sample Enrichment
26
27
Foods
28
(a)
Allow the Demi-Fraser Broth enrichment medium (includes ferric ammonium citrate)
29
to equilibrate to ambient laboratory temperature (20-25
o
C).
30
(b)
Aseptically combine the enrichment medium and sample according to Table 2. For all
31
meat and highly particulate samples, the use of filter bags is recommended.
32
(c)
Homogenize thoroughly by blending, stomaching, or hand mixing for 2 ±0.2 minutes.
33
Incubate at 37 ±1°C according to Table A.
34
35
Environmental samples
36
(d)
Sample collection devices can be a sponge hydrated with a neutralizing solution to
37
inactivate the effects of the sanitizers. 3M recommends the use of a biocide-free
38
cellulose sponge. Neutralizing solution can be Dey-Engley (D/E) Neutralizing Broth
39
or Letheen broth. It is recommended to sanitize the area after sampling.
40
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 A/ Collaboartive Study Manuscript
OMA ERP June 2016
ERP Use Only