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AOAC Official Method 2016.xxx
1
Listeria
speciesin SelectFoods and Environmental Surfaces
2
3M
™
Molecular Detection Assay(MDA) 2-
Listeria
Method
3
First Action 2016
4
5
(Applicable to detection of
Listeria
speciesin hot dogs (25g & 125g), salmon (25g), deli turkey
6
(25g & 125g), cottage cheese (25g), vanilla ice cream (25g), queso fresco (25g), spinach (25g),
7
melon (whole), raw chicken leg pieces (25g), raw chicken fillet (25g); concrete (3M™ Hydrated
8
Sponge Stick with D/E, 225 mL & 100 mL), stainless steel (3M™ Hydrated Sponge Stick with
9
D/E, 225 mL), and plastic (3M™ Enviroswab with Letheen, 10 mL) environmental samples.
10
11
See Tables
2016.1A
and
2016.1B
for a summary of results of the inter-laboratory study.
12
See Tables
2016.2A
and
2016.2B
for detailed results of the inter-laboratory study
13
14
A.
Principle
15
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The 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria
method is used with the 3M
™
17
Molecular Detection System (MDS) for the rapid and specific detection of
Listeria
in enriched
18
food and food process environmental samples. The 3M MDA2 -
Listeria
uses loop-mediated
19
isothermal amplification of unique DNA target sequences with high specificity and sensitivity,
20
combined with bioluminescence to detect the amplification. Presumptive positive results are
21
reported in real-time while negative results are displayed after the assay is completed. Samples
22
arepre-enriched in Demi Fraser broth with ferric ammonium citrate (FAC).
23
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B.
Apparatus and Reagents
25
Items (b)-(g) are available as the 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria
kit
26
from 3M Food Safety (St. Paul, MN 55144-1000, USA).
27
(a)
3M Molecular Detection System
(MDS100) – Available from 3M Food Safety (St.
28
Paul, MN 55144-1000, USA).
29
(b)
3M Molecular Detection Assay 2 – Listeria reagent tubes-
12 strips of 8 tubes.
30
Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
31
(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
32
(d)
Extra caps
– 12 strips of 8 caps
33
(e)
Reagent Control –
8 reagent tubes
34
(f)
Quick Start Guide
35
(g)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
36
(St. Paul, MN 55144-1000, USA).
37
(h)
3M
™
Molecular Detection Chill Block Insert
- Available from 3M
™
Food Safety (St.
38
Paul, MN 55144-1000, USA).
39
(i)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
40
(St. Paul, MN 55144-1000, USA).
41
(j)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
42
Food Safety (St. Paul, MN 55144-1000, USA).
43
(k)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
44
Food Safety (St. Paul, MN 55144-1000, USA).
45
(l)
Empty Lysis Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
46
USA).
47
(m)
Empty Reagent Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-
48
1000, USA).
49
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 A/ Collaboartive Study Manuscript
OMA ERP June 2016
ERP Use Only