102 / 596
6
(n)
Demi Fraser Broth -
Available from 3M Food Safety (St. Paul, MN 55144-1000,
1
USA).
2
(o)
Ferric Ammonium Citrate (FAC),
ACS grade, 5% sterilized
-
Available from MP
3
Biomedicals™or equivalent.
4
(p)
Disposable pipette
– capable of 20 µL
5
(q)
Multi
-
channel (8-channel) pipette
- capable of 20 µL
6
(r)
Sterile filter tip pipette tips
- capable of 20 µL
7
(s)
Filter Stomacher® bags –
Seward or equivalent
.
8
(t)
Stomacher
®– Seward or equivalent.
9
(u)
Thermometer –
calibrated range to include 100 ± 1
o
C
10
(v)
Dry block heater unit–
capable of maintaining 100 ± 1
o
C
11
(w)
Incubators.
– Capable of maintaining 37 ± 1°C or 41.5 ± 1°C.
12
(x)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
13
Detection Chill Block Tray
14
(y)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular Detection
15
Assay components
16
(z)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
17
(aa)
3M™ Enviroswab, --
(hydrated with Letheen) Available from 3M Food
18
Safety(Australia)
19
(bb)
3M™ Hydrated Sponge Stick with 10 mL of D/E –
Available from 3M Food
20
Safety(St. Paul, MN 55144-1000, USA)
21
22
C. General Instructions
23
24
(a)
Store the 3M Molecular Detection Assay 2 -
Listeria
at 2-8°C. Do not freeze. Keep
25
kit away from light during storage. After opening the kit, check that the foil pouch is
26
undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes
27
should always be stored in the re-sealable pouch with the desiccant inside to maintain
28
stability of the lyophilized reagents. Store resealed pouches at 2-8°C for no longer
29
than 60 days. Do not use 3M Molecular Detection Assay 2 -
Listeria
past the
30
expiration date.
31
(b)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
32
Safety Precautions
33
The 3M MDA2 –
Listeria
is intended for use in a laboratory environment by
34
professionals trained in laboratory techniques. 3M has not documented the use of this
35
product in industries other than the food and beverage industries. For example, 3M
36
has not documented this product for testing drinking water, pharmaceutical,
37
cosmetics, clinical or veterinary samples. The 3M MDA2 –
Listeria
has not been
38
evaluated with all possible food products, food processes, testing protocols or with all
39
possible strains of bacteria.
40
As with all test methods, the source of enrichment medium can influence the results.
41
The 3M MDA 2 –
Listeria
has only been evaluated for use with the enrichment media
42
specified in the Instructions for Use section.
43
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 A/ Collaboartive Study Manuscript
OMA ERP June 2016
ERP Use Only