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Evaluation of the 3M
™
Molecular Detection Assay(MDA) 2 -
Listeria
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monocytogenes
for the Detection of
Listeria monocytogenes
in Select
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Foods and Environmental Surfaces: Collaborative Study
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Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins
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Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214
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Lisa Monteroso
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3M Food Safety Department,
3MCenter – Bldg. 260-6B-01, St. Paul, MN 55144
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Collaborators: R. Brooks, J. Walia, F. Hernandez, D. Bosco, G. Trevino, A. Brandt, C. Lopez,
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E. Sjogren, M. Shekhawat, L. Ma, C. Timmons, C. Diaz Proano,A. Calle,Z. Metz, D. Baumler, R.
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Smith, D. Wood, E. Maranan, C. Chavarria,J. Miller, B. Schindler, C. Gies, E. Budge, Z. Geurin, A.
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Repeck, C. Zook, C. Barnes, B. Bastin, D. Isfort
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The 3M™ Molecular Detection Assay (MDA) 2
–Listeria monocytogenes
usesloop-
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mediated isothermal amplification of unique DNA target sequences combined
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with bioluminescence to rapidly detect
Listeria monocytogenes
in a broad range
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of food types and environmental surfaces. Using an unpaired study design,
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technicians from 13 laboratories located in the United States and Canada,
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compared the 3M MDA 2 -
Listeria monocytogenes
to the United States Department
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of Agriculture (USDA) Food Safety Inspection Service (FSIS) Microbiology
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Laboratory Guidebook (MLG) Chapter 8.09
Isolation and Identification of Listeria
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monocytogenes from Red Meat, Poultry and Egg Products, and Environmental
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Samples
reference method for the detection of
Listeria monocytogenes
in deli
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turkey and raw chicken breast fillet. Each matrix was evaluated at three levels of
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contamination: an un-inoculated control level
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(0 colony forming units (CFU)/test portion)
,
a low inoculum level (0.2-2 CFU/test
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portion) and a high inoculum level (2-5 CFU/test portion).Statistical analysis was
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conducted according to the Probability of Detection (POD) statistical
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model.Results obtained for the low inoculum level test portions produced a
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dLPOD value with 95% confidence intervals of 0.04, (-0.08, 0.17) for deli turkey
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indicating no statistically significant difference between the candidate and
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reference methods. For raw chicken breast fillet a dLPOD valuewith 95%
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confidence interval of 0.16, (0.04, 0.28) indicating astatisticallysignificant
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differencebetween the candidate and reference methods with a positive
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correlation in data indicating more recovery of the target analyte by the candidate
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method.
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___________________________________________________________________________
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Listeriamonocytogenes
is a highly pathogenic microorganism that contaminates food and can
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cause non-invasive gastroenteritis, or severe life-threatening illness, referred to as listeriosis [1].
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Although rare, listeriosis is associated with high hospitalization and mortality rates [2].
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Listeriamonocytogenes
is ubiquitous in the environment, often found in soil, water, sewage and
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damp environments, making it difficult to control in the food processing environment [1]. The
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organism’s ability to survive in processing facilities has led to some highly publicized recent
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outbreaks, specifically in ice cream and packaged salads [3]. The 3M
™
Molecular Detection
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AOAC Research I stitute
Expert Review Pan l Use Only
OMAMAN-30 A/ Collaborative Study Manuscript
OMA ERP June 2016
ERP Use Only