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AOAC Official Method 2016.xxx
1
Listeria monocytogenes
in SelectFoods and Environmental Surfaces
2
3M
™
Molecular Detection Assay(MDA) 2-
Listeria monocytogenes
Method
3
First Action 2016
4
5
(Applicable to detection of
Listeria monocytogenes
hot dogs (25g & 125g), salmon (25g), deli
6
turkey (25g & 125g), cottage cheese (25g), chocolate milk (25 mL), vanilla ice cream (25g),
7
queso fresco (25g), romaine lettuce (25g), melon (whole), raw chicken leg pieces (25g); raw
8
chicken breast fillet (25g), concrete (3M™ Hydrated Sponge Stick with Dey-Engley [D/E] , 225
9
mL & 100 mL), stainless steel (3M Hydrated Sponge Stick with D/E, 225 mL), and plastic
10
(3M™ Enviroswab with Letheen, 10 mL) environmental samples.
11
12
See Tables
2016.1A
and
2016.1B
for a summary of results of the inter-laboratory study.
13
See Tables
2016.2A
and
2016.2B
for detailed results of the inter-laboratory study
14
15
A.
Principle
16
17
The 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria monocytogenes
method is used with
18
the 3M
™
Molecular Detection System (MDS) for the rapid and specific detection of
Listeria
19
monocytogenes
in enriched food and food process environmental samples. The 3M MDA2 -
20
Listeria monocytogenes
uses loop-mediated isothermal amplification of unique DNA target
21
sequences with high specificity and sensitivity, combined with bioluminescence to detect the
22
amplification. Presumptive positive results are reported in real-time while negative results are
23
displayed after the assay is completed. Samples arepre-enriched in Demi Fraser with ferric
24
ammonium citrate broth.
25
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B.
Apparatus and Reagents
27
Items (b)-(g) are available as the 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria
28
monocytogenes
kit from 3M Food Safety (St. Paul, MN 55144-1000, USA).
29
(a)
3M Molecular Detection System
(MDS100) – Available from 3M Food Safety (St.
30
Paul, MN 55144-1000, USA).
31
(b)
3M Molecular Detection Assay 2 – Listeria monocytogenes reagent tubes-
12 strips of
32
8 tubes. Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
33
(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
34
(d)
Extra caps
– 12 strips of 8 caps
35
(e)
Reagent Control –
8 reagent tubes
36
(f)
Quick Start Guide
37
(g)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
38
(St. Paul, MN 55144-1000, USA).
39
(h)
3M
™
Molecular Detection Chill Block Insert
- Available from 3M
™
Food Safety (St.
40
Paul, MN 55144-1000, USA).
41
(i)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
42
(St. Paul, MN 55144-1000, USA).
43
(j)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
44
Food Safety (St. Paul, MN 55144-1000, USA).
45
(k)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
46
Food Safety (St. Paul, MN 55144-1000, USA).
47
(l)
Empty Lysis Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
48
USA).
49
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 A/ Collaborative Study Manuscript
OMA ERP June 2016
ERP Use Only