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6
(m)
Empty Reagent Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-
1
1000, USA).
2
(n)
Demi Fraser Broth -
Available from 3M Food Safety (St. Paul, MN 55144-1000,
3
USA).
4
(o)
Ferric Ammonium Citrate (FAC),
ACS grade, 5% sterilized
-
Available from MP
5
Biomedicals™ or equivalent.
6
(p)
Disposable pipette
– capable of 20 µL
7
(q)
Multi
-
channel (8-channel) pipette
- capable of 20 µL
8
(r)
Sterile filter tip pipette tips
- capable of 20 µL
9
(s)
Filter Stomacher® bags –
Seward or equivalent
.
10
(t)
Stomacher
®– Seward or equivalent.
11
(u)
Thermometer –
calibrated range to include 100 ± 1
o
C
12
(v)
Dry block heater unit–
capable of maintaining 100 ± 1
o
C
13
(w)
Incubators.
– Capable of maintaining 37 ± 1°C or 41.5 ± 1°C.
14
(x)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
15
Detection Chill Block Tray
16
(y)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular Detection
17
Assay components
18
(z)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
19
(aa)
3M™ Enviroswab, --
(hydrated with Letheen) Available from 3M Food
20
Safety(Australia)
21
(bb)
3M™ Hydrated Sponge Stick with 10 mL of D/E –
Available from 3M Food
22
Safety(St. Paul, MN 55144-1000, USA)
23
24
C. General Instructions
25
26
(a)
Store the 3M Molecular Detection Assay 2 -
Listeria monocytogenes
at 2-8°C. Do not
27
freeze. Keep kit away from light during storage. After opening the kit, check that the
28
foil pouch is undamaged. If the pouch is damaged, do not use. After opening, unused
29
reagent tubes should always be stored in the re-sealable pouch with the desiccant
30
inside to maintain stability of the lyophilized reagents. Store resealed pouches at 2-
31
8°C for no longer than 60 days. Do not use 3M Molecular Detection Assay 2 -
Listeria
32
monocytogenes
past the expiration date.
33
(b)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
34
Safety Precautions
35
The 3M MDA2 –
Listeria monocytogenes
is intended for use in a laboratory
36
environment by professionals trained in laboratory techniques. 3M has not
37
documented the use of this product in industries other than the food and beverage
38
industries. For example, 3M has not documented this product for testing drinking
39
water, pharmaceutical, cosmetics, clinical or veterinary samples. The 3M MDA2 –
40
Listeria monocytogenes
has not been evaluated with all possible food products, food
41
processes, testing protocols or with all possible strains of bacteria.
42
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 A/ Collaborative Study Manuscript
OMA ERP June 2016
ERP Use Only