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7
As with all test methods, the source of enrichment medium can influence the results.
1
The 3M MDA 2 –
Listeria monocytogenes
has only been evaluated for use with the
2
enrichment media specified in the Instructions for Use section.
3
The 3M™ Molecular Detection instrument is intended for use with samples that have
4
undergone heat treatment during the assay lysis step, which is designed to destroy
5
organisms present in the sample. Samples that have not been properly heat treated
6
during the assay lysis step may be considered a potential biohazard and should NOT
7
be inserted into the 3M MDS instrument.
8
The user should read, understand and follow all safety information in the instructions
9
for the 3M MDS and the 3M MDA 2 –
Listeria monocytogenes
. Retain the safety
10
instructions for future reference.
11
To reduce the risks associated with exposure to chemicals and biohazards:
12
Performpathogen testing in a properly equipped laboratory under the control of
13
trained personnel.Always follow standard laboratory safety practices, including
14
wearing appropriate protective apparel and eye protection while handling reagents
15
and contaminated samples.Avoid contact with the contents of the enrichment media
16
and reagent tubes after amplification. Dispose of enriched samples according to
17
current industry standards.
18
Listeria monocytogenes
is of particular concern for pregnant women, the aged and the
19
infirmed. It is recommended that these concerned groups avoid handling this
20
organism.After use, the enrichment medium and the 3M MDA 2 -
Listeria
21
monocytogenes
tubes can potentially contain pathogenic materials. Periodically
22
decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) with
23
a 1- 5% (v:v in water) household bleach solution or DNA removal solution. When
24
testing is complete, follow current industry standards for the disposal of contaminated
25
waste. Consult the Safety Data Sheet for additional information and local regulations
26
for disposal.
27
To reduce the risks associated with environmental contamination:Follow current
28
industry standards for disposal of contaminated waste.
29
D.
Sample Enrichment
30
31
Foods
32
(a)
Allow the Demi-Fraser Broth enrichment medium (includes ferric ammonium citrate)
33
to equilibrate to ambient laboratory temperature (20-25
o
C).
34
(b)
Aseptically combine the enrichment medium and sample according to Table 2. For all
35
meat and highly particulate samples, the use of filter bags is recommended.
36
(c)
Homogenize thoroughly by blending, stomaching, or hand mixing for 2 ±0.2 minutes.
37
Incubate at 37 ±1°C according to Table A.
38
39
AOAC Research Ins itute
Expert Review Panel Use Only
OMAMAN-30 A/ Collaborative Study Manuscript
OMA ERP June 2016
ERP Use Only