222 / 596
8
Environmental samples
1
(d)
Sample collection devices can be a sponge hydrated with a neutralizing solution to
2
inactivate the effects of the sanitizers. 3M recommends the use of a biocide-free
3
cellulose sponge. Neutralizing solution can be Dey-Engley (D/E) Neutralizing Broth
4
or Letheen broth. It is recommended to sanitize the area after sampling.
5
6
WARNING: Should you select to use Neutralizing Buffer (NB) that contains aryl
7
sulfonate complex as the hydrating solution for the sponge, it is required to perform a
8
1:2 dilution (1-part sample into 1-part sterile enrichment broth) of the enriched
9
environmental sample before testing in order to reduce the risks associated with a
10
false-negative result leading to the release of contaminated product. Another option is
11
to transfer 10 µL of the NB enrichment in the LS tubes.
12
13
(e)
The recommended size of the sampling area to verify the presence or absence of the
14
pathogen on the surface is at least 100 cm
2
(10 cm x 10 cm or 4”x4”). When sampling
15
with a sponge, cover the entire area going in two directions (left to right then up and
16
down) or collect environmental samples following your current sampling protocol or
17
according to the FDA BAM, USDA FSIS MLG or ISO 18593 [11] guidelines.
18
1.
Allow the Demi-Fraser Broth enrichment medium (includes ferric ammonium
19
citrate) to equilibrate to ambient laboratory temperature (20-25
o
C).
20
2.
Aseptically combine the enrichment medium and sample according to Table 2.
21
3.
Homogenize thoroughly by blending, stomaching, or hand mixing for 2 ±0.2
22
minutes. Incubate at 37 ±1°C for 24-30hours.
23
24
E.
P
REPARATION OF THE
3M™M
OLECULAR
D
ETECTION
S
PEED
L
OADER
T
RAY
25
(a)
Wet a cloth or paper towel with a 1-5% (v:v in water) household bleach solution and
26
wipe the 3M™ Molecular Detection Speed Loader Tray.
27
(b)
Rinse the 3M Molecular Detection Speed Loader Tray with water.
28
(c)
Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
29
(d)
Ensure the 3M Molecular Detection Speed Loader Tray is dry before use
30
31
Specific Instructions for Validated Methods
32
AOAC® Performance Tested Method
sm
# 081501
33
34
35
36
In AOAC PTM studies, the 3M Molecular Detection Assay 2 –
Listeria monocytogenes
37
was found to be an effective method for the detection of
Listeria monocytogenes.
The
38
AOAC Research Institute
Expert R view Panel Use Only
OMAMAN-30 A/ Collaborative Study Manuscript
OMA ERP June 2016
ERP Use Only