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3M MDA 2 Listeria species and L. monocytogenes Collaborative Study
OMA-2016-MONTH-XXX
5/4/16
Lisa Monteroso
Study Director
3M Food Safety Department
3M Center, Building 260-6B-01
St. Paul, MN 55144
Phone: 651-736-2913
Fax: 651-733-5819
Email:
Patrick Bird & Erin Crowley
Co-Study Directors
Q Laboratories, Inc.
1400 Harrison Avenue
Cincinnati, Ohio 45214
Phone : 513-471-1300
Fax : 513-471-5600
Email:
2.0 Collaborators
A total of 15-16 collaborators will be solicited to participate in this study. A minimum of
10 laboratories, with acceptable data, will be needed for the successful completion of this
study. Laboratories currently using the 3M MDS or those who will be thoroughly trained
by a 3M representative, with a clear understanding of the reference methods, will act as
collaborators for this study.
A sample letter to collaborators can be found in Appendix 9.1. This letter will be sent to
each collaborator prior to the start of the study.
3.0 Collaborative Study Design
3.1 This collaborative study is designed according to the AOAC INTERNATIONAL
Methods Committee Guidelines for Validation of Microbiological Methods for
Food and Environmental Surfaces (2012).
3.2 In this study, two food types will be analyzed:
3.2.1 Deli turkey (125g) – 3M MDA 2 -Listeria and 3M MDA 2 - Listeria
monocytogenes compared to USDA/FSIS MLG 8.0
3.2.2 Raw chicken breast (25g) – 3M MDA 2 -Listeria and 3M MDA 2 -
Listeria monocytogenes compared to USDA/FSIS MLG 8.09
3.3 Collaborators will receive test portions for each matrix:
3.3.1 Raw chicken breast – 36 x 60g samples. There will be 12 uninoculated
control, 12 low level (target 0.2 – 2.0 cfu/test portion), and 12 high level
(target 2 – 5 cfu/test portion) samples. Each sample will be divided into
two separate 25g test portions: one will be tested using the 3M MDA 2test
kits (Listeria and Listeria monocytogenes) and the other will be tested by
the reference method.
3.3.2 Deli turkey – 72 x 125g samples. There will be 24 uninoculated control,
24 low level (target 0.2 – 2.0 cfu/test portion), and 24 high level (target 2 –
5 cfu/test portion) samples. Thirty-six 125g test portions (12 at each level)
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only