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3M MDA 2
Listeria
species and
L. monocytogenes
Collaborative Study
OMA-2016-MONTH-XXX
5/4/16
4.1.10
Raw chicken fillet
(25 g): 28-32 hours of enrichment in Demi Fraser
broth at 37°C
4.1.11
Stainless steel
: sponge in 225 mL with 24-30 hours of enrichment in
Demi Fraser broth at 37°C
4.1.12
Concrete
: sponge in 100 mL and 225 mL and sponge in 225 mL with24-
30 hours of enrichment in Demi Fraser broth at 37°C
4.1.13
Plastic
: Enviroswab in 10 mL 24-30 hours of enrichment in Demi Fraser
broth at 37°C
Caution
:
The 3M MDA2 -
Listeria
is intended for use in a laboratory
environment by professionals trained in laboratory techniques. The user
should read, understand and follow all safety information in the instructions
for the 3M MDS and the 3M MDA2 -
Listeria
and retain the safety
instructions for future reference.
The 3M MDA 2 -
Listeria
method may generate
Listeria monocytogenes
to
levels sufficient to cause stillbirths and fatalities in pregnant women and the
immuno compromised, if exposed. The user must train its personnel in
current proper testing techniques: for example, Good Laboratory Practices,
ISO 17025, or ISO 7218.
Follow all instructions carefully. Failure to do so may lead to inaccurate
results. The 3M Molecular Detection Instrument is intended for use with
samples that have undergone heat treatment during the assay lysis step, which
is designed to destroy organisms present in the sample. Samples that have not
been properly heat treated during the assay lysis step may be considered a
potential biohazard and should NOT be inserted into the 3M MDS
(instrument).
Store the 3M MDA2 -
Listeria
at 2-8°C. Do not freeze. Keep kit away from
light during storage. After opening the kit, check that the foil pouch is
undamaged. If the pouch is damaged, do not use. After opening, unused
reagent tubes should always be stored in the resealable pouch with the
desiccant inside to maintain stability of the lyophilized reagents. Store
resealed pouches at 2-8°C for no longer than 1 month. Do not use 3M
MDA2 -
Listeria
past the expiration date. Expiration date and lot number are
noted on the outside label of the box.
After use, the enrichment medium and the 3M MDA2 -
Listeria
tubes can
potentially contain pathogenic materials. When testing is complete, follow
current industry standards for the disposal of contaminated waste. Consult the
local regulations for disposal.
4.2
Test Kit Reagents -
3M MDA 2 -
Listeria
- 96 tests
4.2.1
Lysis Solution (LS) tubes
. – 96 (12 strips of 8 tubes)
4.2.2
Listeria Reagent tubes
. – 96 (12 strips of 8 tubes)
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only