AOAC-RI ERP Book MICRO.pdf

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3M MDA 2 Listeria species and L. monocytogenes Collaborative Study
OMA-2016-MONTH-XXX
5/4/16
will be tested using the 3M MDA 2 test kits (Listeria and Listeria
monocytogenes) and thirty-six 125g test portions (12 at each level) will be
tested by the reference method.
3.3.3 Each laboratory will test all test portions. Each food will be inoculated
with a different Listeria monocytogenes strain as listed in Table 1 and will
be randomized and blind-coded for analysis.
3.4 Study Schedule
3.4.1 One matrix will be tested each week of the study. All data should be sent
to the Study Director by the 4th week of study.
3.4.2 The contract laboratory will prepare the test portions and ship the test
portions refrigerated via overnight courier to the collaborators to arrive on
a Friday. Test portion processing and analysis will begin on Monday.
Data forms will be completed and faxed or emailed back to the co-Study
Director by the following Friday.
Table 1. Overview of Study Design
MATRIX Inoculating
organism
Target L.
monocytogenes
Levels
Test Portion
Codes
Reference
Method
Raw chicken
breast
25g – 3M MDA2
25g – USDA/FSIS
MLG
L. monocytogenes
TBD
0 cfu/test portion Random
number
between
200-299
USDA/FSIS
MLG 8.09 Rev.
09 (5/1/2013)
0.2-2 cfu/test portion
2-5 cfu/test portion
Deli turkey
125g – 3M MDA2
125g –
USDA/FSIS MLG
L. monocytogenes
TBD
0 cfu/test portion Random
number
between
300-399
USDA/FSIS
MLG 8.09 Rev.
09 (5/1/2013)
0.2-2 cfu/test portion
2-5 cfu/test portion AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only