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3M MDA 2
Listeria
species and
L. monocytogenes
Collaborative Study
OMA-2016-MONTH-XXX
5/4/16
4.6.14
Confirmation
All positive 3M MDA2 -
Listeria
results must be confirmed. Confirmation
should be performed using the primary enrichment, carried through as
described in the reference method starting with the secondary transfer and
selective isolation. Confirmation should be initiated within 72 hrs. Isolate
on a selective agar and identify 1 to 5 typical colonies according to the
conventional tests described in the AOAC method.
5.0
3M MDA 2 -
Listeria monocytogenes
5.1
Applicability
For the detection of
Listeria monocytogenes
from:
5.1.1
Beef hot dogs
(25 g and 125 g): 24-30 hours of enrichment in Demi Fraser
broth at 37°C
5.1.2
Cold smoked salmon
(25 g): 24-30 hours of enrichment in Demi Fraser
broth at 37°C
5.1.3
Deli turkey
(25 g and 125 g): 24-30 hours of enrichment in Demi Fraser
broth at 37°C
5.1.4
Full fat cottage cheese
(25 g): 24-30 hours of enrichment in Demi Fraser
broth at 37°C
5.1.5
Queso fresco
(25 g): 24-30 hours of enrichment in Demi Fraser broth at
37°C
5.1.6
Chocolate milk
(25 mL): 24-30 hours of enrichment in Demi Fraser broth
at 37°C
5.1.7
Vanilla ice cream
(25 g): 24-30 hours of enrichment in Demi Fraser broth
at 37°C
5.1.8
Romaine lettuce
(25 g): 24-30 hours of enrichment in Demi Fraser broth
at 37°C
5.1.9
Cantaloupe
(whole melon): 24-30 hours of enrichment in Demi Fraser
broth at 37°C
5.1.10
Raw chicken leg pieces
(25 g): 28-32 hours of enrichment in Demi Fraser
broth at 37°C
5.1.11
Stainless steel
: sponge in 225 mL with 24-30 hours of enrichment in
Demi Fraser broth at 37°C
5.1.12
Concrete
: sponge in 100 mL and 225 mL and sponge in 225 mL with24-
30 hours of enrichment in Demi Fraser broth at 37°C
5.1.13
Plastic
: Enviroswab in 10 mL 24-30 hours of enrichment in Demi Fraser
broth at 37°C
Caution
:
The 3M MDA 2 -
Listeria monocytogenes
is intended for use in a
laboratory environment by professionals trained in laboratory techniques. The
user should read, understand and follow all safety information in the
instructions for the 3M MDS and the 3M MDA 2 -
Listeria monocytogenes
and retain the safety instructions for future reference. Follow all instructions
carefully. Failure to do so may lead to inaccurate results.
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only