32 / 596
3M MDA 2
Listeria
species and
L. monocytogenes
Collaborative Study
OMA-2016-MONTH-XXX
5/4/16
4.2.3
Extra caps.
– 96 (12 strips of 8 caps)
4.2.4
Reagent Control (RC).
– 16 (2 pouches of 8)
4.2.5
Quick Start Guide
4.3
Additional Supplies and Reagents
4.3.1
3M Molecular Detection System (instrument), power supply, cord,
USB cable and software
4.3.2
3M Molecular Detection Speed Loader Tray
4.3.3
3M Molecular Detection Chill Block Tray and Chill block insert
4.3.4
3M Molecular Detection Heat Block Insert
4.3.5
3M Molecular Detection Cap/Decap Tool [Reagent]
4.3.6
3M Molecular Detection Cap/Decap Tool [Lysis]
4.3.7
Empty lysis tube rack
4.3.8
Empty reagent tube rack
4.3.9
Dey-Engley (D/E) Neutralizing Broth
4.3.10
Demi-Fraser broth
4.4
Additional media
4.4.1
Oxford Agar (OX)
4.4.2
Sheep blood agar (SBA)
4.5
Apparatus
4.5.1
Pipettes
. – capable of 20µL
4.5.2
Multi-channel pipette
. – capable of 20µL
4.5.3
Sterile pipette tips.
– capable of 20µL
4.5.4
Stomacher®
. – Seward or equivalent
4.5.5
Filter Stomacher® bags
. - Seward or equivalent
4.5.6
Thermometer
. – calibrated range to include 100 ± 1°C range
4.5.7
Incubators
. – capable of maintaining 37 ± 1°C
4.5.8
Dry double block heater unit.
– capable of maintaining 100 ± 1°C;
OR
a water bath
capable of maintaining 100 ± 1°C
4.5.9
Freezer
. – capable of maintaining -10 to -20°C, for storing the 3M
Molecular Detection Chill Block Tray
4.5.10
Refrigerator
. – capable of maintaining 2-8°C, for storing the 3M
MDA
4.5.11
Computer
. – compatible with the 3M Molecular Detection System
(instrument)
4.6
Determination
4.6.1
Enrichment
4.6.1.1
Deli turkey
- aseptically add the 125g test portion to 1125 mL
Demi Fraser broth.
4.6.1.1.1
Homogenize thoroughly in a filter bag for 2 minutes ±
30 seconds
4.6.1.1.2
Incubate 24-30 hr at 37 ± 1°C.
AOAC Rese rch Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only