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inoculum levels for each environmental surface are presented in Table 7. A summary of POD
1
analyses [13] are presented in Tables 8-15. As per criteria outlined in Appendix J of the Official
2
Methods of Analysis Manual [13], fractional positive results were obtained for all matrices.
3
4
Internal Study
5
6
Raw Chicken Leg Pieces (25 g) – 28 hour Primary Enrichment
7
8
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 10
9
presumptive positives and 10 confirmed positives following the USDA/FSIS MLG 8.09
10
reference method confirmation procedure. There were 12 observed positives for the reference
11
method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed
12
positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There
13
were 5 confirmed positive following the reference method.Detailed results of the POD analyses
14
are presented in Tables 8 and 12.
15
16
Bagged raw spinach
17
18
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 9
19
presumptive positives and 10 confirmed positives following the ISO 11290-1/A1 reference
20
method confirmation procedure resulting in 1 false negative. There were 10 observed positives
21
for the reference method. For the high inoculation level, there were 5 presumptive positives and
22
5 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure.
23
There were 4 confirmed positives following the reference method.Detailed results of the POD
24
analyses are presented in Tables 8 and 12.
25
26
27
Cold smoked salmon
28
29
Compared to FDA-BAM
30
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 10
31
presumptive positives and 10 confirmed positives following the FDA/BAM Chapter 10 reference
32
method confirmation procedure. There were 7 observed positives for the reference method. For
33
the high inoculation level, there were 3 presumptive positives and 3 confirmed positives
34
following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 5
35
confirmed positives following the reference method.Detailed results of the POD analyses are
36
presented in Tables 8 and 12.
37
38
Compared to ISO 11290-1/A1
39
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 10
40
presumptive positives and 10 confirmed positives following the ISO 11290-1/A1 reference
41
method confirmation procedure. There were 9 observed positives for the reference method. For
42
the high inoculation level, there were 3 presumptive positives and 3 confirmed positives
43
following the ISO 11290-1/A1 reference method confirmation procedure. There were 4
44
confirmed positives following the reference method.Detailed results of the POD analyses are
45
presented in Tables 8 and 12.
46
AOAC Research Institute
Exp rt Review Panel Use Only
OMAMAN-30 D/ PTM Validation Report 081501
OMA ERP - June 2016
ERP Use Only