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For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 4
1
presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference
2
method confirmation procedure, resulting in 1 false negative. There were 6 observed positives
3
for the reference method. For the high inoculation level, there were 5 presumptive positives and
4
5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
5
procedure. There were 5 confirmed positives following the reference method. Detailed results of
6
the POD analyses are presented in Tables 10 and 14.
7
8
Stainless Steel (enriched in 225 mL)
9
10
Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level
11
of the MDA2 -
Listeria monocytogenes
assay, there were 5 presumptive positives and 5
12
confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
13
procedure. There were 8 observed positives for the reference method. For the high inoculation
14
level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS
15
MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives
16
following the reference method.Detailed results of the POD analyses are presented in Tables 11
17
and 15.
18
19
Sealed Concrete (enriched in 100 mL)
20
21
24-hour primary enrichment
- For the low inoculation level of the MDA2 –
22
Listeriamonocytogenes
assay, there were 15 presumptive positives and 15 confirmed positives
23
following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 11
24
observed positives for the reference method. For the high inoculation level, there were 5
25
presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference
26
method confirmation procedure. There were 5 confirmed positives following the reference
27
method.Detailed results of the POD analyses are presented in Tables 11 and 15.
28
29
26-hour primary enrichment
- For the low inoculation level of the MDA2 –
30
Listeriamonocytogenes
assay, there were 16 presumptive positives and 15 confirmed positives
31
following the USDA/FSIS MLG 8.09 reference method confirmation procedure, resulting in 1
32
false positive. There were 11 observed positives for the reference method. For the high
33
inoculation level, there were 5 presumptive positives and 5 confirmed positives following the
34
USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed
35
positives following the reference method.Detailed results of the POD analyses are presented in
36
Tables 11 and 15.
37
38
Plastic (enriched in 10 mL)
39
40
Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level
41
of the MDA2 -
Listeria monocytogenes
assay, there were 12 presumptive positives and 12
42
confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
43
procedure. There were 10 observed positives for the reference method. For the high inoculation
44
level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS
45
MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives
46
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 D/ PTM Validation Report 081501
OMA ERP - June 2016
ERP Use Only