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For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 4

1

presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference

2

method confirmation procedure, resulting in 1 false negative. There were 6 observed positives

3

for the reference method. For the high inoculation level, there were 5 presumptive positives and

4

5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation

5

procedure. There were 5 confirmed positives following the reference method. Detailed results of

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the POD analyses are presented in Tables 10 and 14.

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8

Stainless Steel (enriched in 225 mL)

9

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Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level

11

of the MDA2 -

Listeria monocytogenes

assay, there were 5 presumptive positives and 5

12

confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation

13

procedure. There were 8 observed positives for the reference method. For the high inoculation

14

level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS

15

MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives

16

following the reference method.Detailed results of the POD analyses are presented in Tables 11

17

and 15.

18

19

Sealed Concrete (enriched in 100 mL)

20

21

24-hour primary enrichment

- For the low inoculation level of the MDA2 –

22

Listeriamonocytogenes

assay, there were 15 presumptive positives and 15 confirmed positives

23

following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 11

24

observed positives for the reference method. For the high inoculation level, there were 5

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presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference

26

method confirmation procedure. There were 5 confirmed positives following the reference

27

method.Detailed results of the POD analyses are presented in Tables 11 and 15.

28

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26-hour primary enrichment

- For the low inoculation level of the MDA2 –

30

Listeriamonocytogenes

assay, there were 16 presumptive positives and 15 confirmed positives

31

following the USDA/FSIS MLG 8.09 reference method confirmation procedure, resulting in 1

32

false positive. There were 11 observed positives for the reference method. For the high

33

inoculation level, there were 5 presumptive positives and 5 confirmed positives following the

34

USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed

35

positives following the reference method.Detailed results of the POD analyses are presented in

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Tables 11 and 15.

37

38

Plastic (enriched in 10 mL)

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Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level

41

of the MDA2 -

Listeria monocytogenes

assay, there were 12 presumptive positives and 12

42

confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation

43

procedure. There were 10 observed positives for the reference method. For the high inoculation

44

level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS

45

MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives

46

AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-30 D/ PTM Validation Report 081501

OMA ERP - June 2016

ERP Use Only