AOAC-RI ERP Book MICRO.pdf

Table 1. Enrichment Protocols Using Demi-Fraser Broth Enrichment

at 37 ± 1 °C

Sample Matrix

Sample

Size

Enrichment

Broth Volume

(mL)

Enrichment

Time (hr)

Beef hot dogs, Queso Fresco,

Vanilla Ice Cream, 4% Milk Fat

Cottage Cheese, 3% chocolate

whole milk, romaine lettuce, bagged

raw spinach, cold smoked salmon

25 g

225

24-30

Deli Turkey

125 g

1175

24-30

Environm

ental

samples:

stainless steel,

sealed concrete

1 sponge

100 or 225

24-30

Plastic,

1 swab

24-30

Raw Chicken

25 g

475

28-32

AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-30 D/ PTM Validation Report 081501

OMA ERP - June 2016

ERP Use Only