following the reference method.Detailed results of the POD analyses are presented in Tables 9
1
and 13.
2
3
Romaine lettuce (25 g)
4
5
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 7
6
presumptive positives and 7 confirmed positives following the FDA/BAM Chapter 10 reference
7
method confirmation procedure. There were 6 observed positives for the reference method. For
8
the high inoculation level, there were 5 presumptive positives and 5 confirmed positives
9
following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 5
10
confirmed positives following the reference method.Detailed results of the POD analyses are
11
presented in Tables 10 and 14.
12
13
Queso Fresco (25 g)
14
15
For the low inoculation level of the MDA2 –
Listeriamonocytogenes
assay, there were 11
16
presumptive positives and 11 confirmed positives following the AOAC 993.12 reference method
17
confirmation procedure. There were 9 observed positives for the reference method. For the high
18
inoculation level, there were 5 presumptive positives and 5 confirmed positives following the
19
AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives
20
following the reference method.Detailed results of the POD analyses are presented in Tables 10
21
and 14.
22
23
4%Milk Fat Cottage Cheese (25 g)
24
25
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 10
26
presumptive positives and 10 confirmed positives following the AOAC 993.12 reference method
27
confirmation procedure. There were 8 observed positives for the reference method. For the high
28
inoculation level, there were 5 presumptive positives and 5 confirmed positives following the
29
AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives
30
following the reference method.Detailed results of the POD analyses are presented in Tables 10
31
and 14.
32
33
3% Chocolate Whole Milk (25 mL)
34
35
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 9
36
presumptive positives and 9 confirmed positives following the AOAC 993.12 reference method
37
confirmation procedure. There were 6 observed positives for the reference method. For the high
38
inoculation level, there were 5 presumptive positives and 5 confirmed positives following the
39
AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives
40
following the reference method.Detailed results of the POD analyses are presented in Tables 10
41
and 14.
42
43
Beef Hot Dog (25 g)
44
45
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 D/ PTM Validation Report 081501
OMA ERP - June 2016
ERP Use Only