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following the reference method.Detailed results of the POD analyses are presented in Tables 9

1

and 13.

2

3

Romaine lettuce (25 g)

4

5

For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 7

6

presumptive positives and 7 confirmed positives following the FDA/BAM Chapter 10 reference

7

method confirmation procedure. There were 6 observed positives for the reference method. For

8

the high inoculation level, there were 5 presumptive positives and 5 confirmed positives

9

following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 5

10

confirmed positives following the reference method.Detailed results of the POD analyses are

11

presented in Tables 10 and 14.

12

13

Queso Fresco (25 g)

14

15

For the low inoculation level of the MDA2 –

Listeriamonocytogenes

assay, there were 11

16

presumptive positives and 11 confirmed positives following the AOAC 993.12 reference method

17

confirmation procedure. There were 9 observed positives for the reference method. For the high

18

inoculation level, there were 5 presumptive positives and 5 confirmed positives following the

19

AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives

20

following the reference method.Detailed results of the POD analyses are presented in Tables 10

21

and 14.

22

23

4%Milk Fat Cottage Cheese (25 g)

24

25

For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 10

26

presumptive positives and 10 confirmed positives following the AOAC 993.12 reference method

27

confirmation procedure. There were 8 observed positives for the reference method. For the high

28

inoculation level, there were 5 presumptive positives and 5 confirmed positives following the

29

AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives

30

following the reference method.Detailed results of the POD analyses are presented in Tables 10

31

and 14.

32

33

3% Chocolate Whole Milk (25 mL)

34

35

For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 9

36

presumptive positives and 9 confirmed positives following the AOAC 993.12 reference method

37

confirmation procedure. There were 6 observed positives for the reference method. For the high

38

inoculation level, there were 5 presumptive positives and 5 confirmed positives following the

39

AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives

40

following the reference method.Detailed results of the POD analyses are presented in Tables 10

41

and 14.

42

43

Beef Hot Dog (25 g)

44

45

AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-30 D/ PTM Validation Report 081501

OMA ERP - June 2016

ERP Use Only