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1

2

Independent Study

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4

Deli Turkey (125 g)

5

6

For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 8

7

presumptive positives and 8 confirmed positives following the USDA/FSIS MLG 8.09 reference

8

method confirmation procedure. There were 7 observed positives for the reference method. For

9

the high inoculation level, there were 5 presumptive positives and 5 confirmed positives

10

following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5

11

confirmed positives following the reference method.Detailed results of the POD analyses are

12

presented in Tables 9 and 13.

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14

Raw Chicken Leg Pieces (25 g)

15

16

Lot 1

17

For the lot 1 of the raw chicken leg pieces of the MDA2 -

Listeria monocytogenes

assay, there

18

were 17 presumptive positives and 17 confirmed positives following the USDA/FSIS MLG 8.09

19

reference method confirmation procedure. There were 16 observed positives for the reference

20

method. Detailed results of the POD analyses are presented in Tables 9 and 13.

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22

Lot 2

23

For the lot 2 of the raw chicken leg pieces of the MDA2 -

Listeriamonocytogenes

assay, there

24

were 10 presumptive positives and 10 confirmed positives following the USDA/FSIS MLG 8.09

25

reference method confirmation procedure. There were 14 observed positives for the reference

26

method. Detailed results of the POD analyses are presented in Tables 9 and 13.

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28

Whole Melon

29

30

For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 10

31

presumptive positives and 10 confirmed positives following the FDA/BAM Chapter 10 reference

32

method confirmation procedure. There were 8 observed positives for the reference method. For

33

the high inoculation level, there were 5 presumptive positives and 5 confirmed positives

34

following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 5

35

confirmed positives following the reference method.Detailed results of the POD analyses are

36

presented in Tables 9 and 13.

37

38

39

Vanilla Ice Cream (25 g)

40

41

For the low inoculation level of the MDA2 -

Listeria monocytogenes

assay, there were 7

42

presumptive positives and 7 confirmed positives following the AOAC 993.12 reference method

43

confirmation procedure. There were 7 observed positives for the reference method. For the high

44

inoculation level, there were 5 presumptive positives and 5 confirmed positives following the

45

AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives

46

AOAC Research Institute

Exp rt Review Panel Use Only

OMAMAN-30 D/ PTM Validation Report 081501

OMA ERP - June 2016

ERP Use Only