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AOAC Official Method 2016.xxx
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Listeria
speciesin SelectFoods and Environmental Surfaces
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3M
™
Molecular Detection Assay(MDA) 2-
Listeria
Method
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First Action 2016
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(Applicable to detection of
Listeria
speciesin hot dogs (25g & 125g), salmon (25g), deli turkey
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(25g & 125g), cottage cheese (25g), vanilla ice cream (25g), queso fresco (25g), spinach (25g),
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melon (whole), raw chicken leg pieces (25g), raw chicken fillet (25g); concrete (3M™ Hydrated
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Sponge Stick with D/E, 225 mL & 100 mL), stainless steel (3M™ Hydrated Sponge Stick with
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D/E, 225 mL), and plastic (3M™ Enviro
sS
wab with Letheen, 10 mL) environmental samples.
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See Tables
2016.1A
and
2016.1B
for a summary of results of the inter-laboratory study.
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See Tables
2016.2A
and
2016.2B
for detailed results of the inter-laboratory study
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A.
Principle
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The 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria
method is used with the 3M
™
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Molecular Detection System (MDS) for the rapid and specific detection of
Listeria
in enriched
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food and food process environmental samples. The 3M MDA2 -
Listeria
uses loop-mediated
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isothermal amplification of unique DNA target sequences with high specificity and sensitivity,
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combined with bioluminescence to detect the amplification. Presumptive positive results are
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reported in real-time while negative results are displayed after the assay is completed. Samples
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arepre-enriched in Demi Fraser broth with ferric ammonium citrate (FAC).
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B.
Apparatus and Reagents
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Items (b)-(g) are available as the 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria
kit
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from 3M Food Safety (St. Paul, MN 55144-1000, USA).
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(a)
3M Molecular Detection System
(MDS100) – Available from 3M Food Safety (St.
34
Paul, MN 55144-1000, USA).
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(b)
3M Molecular Detection Assay 2 – Listeria reagent tubes-
12 strips of 8 tubes.
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Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
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(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
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(d)
Extra caps
– 12 strips of 8 caps
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(e)
Reagent Control –
8 reagent tubes
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(f)
Quick Start Guide
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(g)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
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(St. Paul, MN 55144-1000, USA).
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(h)
3M
™
Molecular Detection Chill Block Insert
- Available from 3M
™
Food Safety (St.
44
Paul, MN 55144-1000, USA).
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(i)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
46
(St. Paul, MN 55144-1000, USA).
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(j)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
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Food Safety (St. Paul, MN 55144-1000, USA).
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