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7
evaluated with all possible food products, food processes, testing protocols or with all
1
possible strains of bacteria.
2
As with all test methods, the source of enrichment medium can influence the results.
3
The 3M MDA 2 –
Listeria
has only been evaluated for use with the enrichment media
4
specified in the Instructions for Use section.
5
The 3M™ Molecular Detection instrument is intended for use with samples that have
6
undergone heat treatment during the assay lysis step, which is designed to destroy
7
organisms present in the sample. Samples that have not been properly heat treated
8
during the assay lysis step may be considered a potential biohazard and should NOT
9
be inserted into the 3M MDS instrument.
10
The user should read, understand and follow all safety information in the instructions
11
for the 3M MDS and the 3M MDA 2 –
Listeria
. Retain the safety instructions for
12
future reference.
13
To reduce the risks associated with exposure to chemicals and biohazards:
14
Performpathogen testing in a properly equipped laboratory under the control of
15
trained personnel.Always follow standard laboratory safety practices, including
16
wearing appropriate protective apparel and eye protection while handling reagents
17
and contaminated samples.Avoid contact with the contents of the enrichment media
18
and reagent tubes after amplification.Dispose of enriched samples according to
19
current industry standards.
20
Listeria monocytogenes
is of particular concern for pregnant women, the aged and the
21
infirmed. It is recommended that these concerned groups avoid handling this
22
organism.After use, the enrichment medium and the 3M MDA 2 -
Listeria
tubes can
23
potentially contain pathogenic materials.Periodically decontaminate laboratory
24
benches and equipment (pipettes, cap/decap tools, etc.) with a 1- 5% (v:v in water)
25
household bleach solution or DNA removal solution. When testing is complete,
26
follow current industry standards for the disposal of contaminated waste. Consult the
27
Safety Data Sheet for additional information and local regulations for disposal.
28
To reduce the risks associated with environmental contamination:Follow current
29
industry standards for disposal of contaminated waste.
30
D.
Sample Enrichment
31
32
Foods
33
(a)
Allow the Demi-Fraser Broth enrichment medium (includes ferric ammonium citrate)
34
to equilibrate to ambient laboratory temperature (20-25
o
C).
35
(b)
Aseptically combine the enrichment medium and sample according to Table
2A
. For
36
all meat and highly particulate samples, the use of filter bags is recommended.
37
(c)
Homogenize thoroughly by
blending,
stomaching, or hand mixing for 2 ±0.2 minutes.
38
Incubate at 37 ±1°C according to Table A.
39