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6
(k)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
1
Food Safety (St. Paul, MN 55144-1000, USA).
2
(l)
Empty Lysis Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
3
USA).
4
(m)
Empty Reagent Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-
5
1000, USA).
6
(n)
Demi Fraser Broth -
Available from 3M Food Safety (St. Paul, MN 55144-1000,
7
USA).
8
(o)
Ferric Ammonium Citrate (FAC),
ACS grade, 5% sterilized
-
Available from MP
9
Biomedicals™or equivalent.
10
(p)
Disposable pipette
– capable of 20 µL
11
(q)
Multi
-
channel (8-channel) pipette
- capable of 20 µL
12
(r)
Sterile filter tip pipette tips
- capable of 20 µL
13
(s)
Filter Stomacher® bags –
Seward or equivalent
.
14
(t)
Stomacher
®– Seward or equivalent.
15
(u)
Thermometer –
calibrated range to include 100 ± 1
o
C
16
(v)
Dry block heater unit–
capable of maintaining 100 ± 1
o
C
17
(w)
Incubators.
– Capable of maintaining 37 ± 1°C
or 41.5 ± 1°C.
18
(x)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
19
Detection Chill Block Tray
20
(y)(x)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular
21
Detection Assay components
22
(z)(y)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
23
(aa)(z)
3M™ Enviro
sS
wab, --
(hydrated with Letheen) Available from 3M Food
24
Safety(Australia)
25
(bb)(aa)
3M™ Hydrated Sponge Stick with 10 mL of D/E –
Available from 3M
26
Food Safety(St. Paul, MN 55144-1000, USA)
27
28
C. General Instructions
29
30
(a)
Store the 3M Molecular Detection Assay 2 -
Listeria
at 2-8°C. Do not freeze. Keep
31
kit away from light during storage. After opening the kit, check that the foil pouch is
32
undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes
33
should always be stored in the re-sealable pouch with the desiccant inside to maintain
34
stability of the lyophilized reagents. Store resealed pouches at 2-8°C for no longer
35
than 60 days. Do not use 3M Molecular Detection Assay 2 -
Listeria
past the
36
expiration date.
37
(b)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
38
Safety Precautions
39
The 3M MDA2 –
Listeria
is intended for use in a laboratory environment by
40
professionals trained in laboratory techniques. 3M has not documented the use of this
41
product in industries other than the food and beverage industries. For example, 3M
42
has not documented this product for testing drinking water, pharmaceutical,
43
cosmetics, clinical or veterinary samples. The 3M MDA2 –
Listeria
has not been
44