404 / 596
St. Paul, MN 55144-1000
1
2
Independent Laboratory
3
Q Laboratories, Inc.
4
1400 Harrison Ave
5
Cincinnati, OH 45214
6
7
Reviewers
8
Yvonne Salfinger
9
Consultant
10
Denver, CO
11
12
Michael Brodsky
13
Brodsky Consultants
14
73 Donnamora Crescent
15
Thornhill, Ontario L3T 4K6
16
Canada
17
18
Reviewer
19
Tony Hitchins
20
FDA-Retired
21
Rockville, MD
22
23
Scope of Method
24
25
a)
Target organism
.
— Listeria
species
(
L. monocytogenes, L. seeligeri, L. ivanovii, L. innocua,
26
L. grayi, L. welshimeri, L. fleishmanii
subsp
.
coloradensis
, L. marthii
,
L. grandensis, L.
27
cornellensis L. aquatic, L. newyorkensis, L. weihenstephanesis, L. baoriae, L. fleishmanii
28
subsp. fleishmanii,
L. ivanovii
subsp. londoniensis and
L. riparia)
(
L. monocytogenes, L.
29
seeligeri, L. ivanovii, L. innocua, L. grayi, L. welshimeri, L. fleishmanii
subsp
. coloradensis,
30
L. cornellensis, L. grandensis
L. marthii
, and
L. riparia)
31
32
b)
Matrixes
.
—
Raw chicken (leg pieces and fillets) (25 g), bagged raw spinach (25 g), cold
33
smoked salmon (25 g), deli turkey (125 g), whole melon, vanilla ice cream (25 g), queso
34
fresco (25 g), 4% milk fat cottage cheese (25 g), beef hot dogs (25 g), stainless steel (4” x 4”,
35
sponge enriched in 225 mL), sealed concrete (4” x 4”, sponge enriched in 225 mL), plastic
36
(1” x 1”, 3M™
Tecra™
Enviro
Ss
wab [3M Australia Pty Ltd] enriched in 10 mL).
37
38
c)
Summary of Validated Performance Claims
.
—
Performance equivalent to that of the U.S.
39
Food and Drug Administration
Bacteriological Analytical Manual
(FDA/BAM) Chapter 10
40
[1] for whole melon, cold smoked salmon, bagged raw spinach; the U.S. Department of
41
Agriculture Food Safety and Inspection Service
Microbiology Laboratory Guidebook
42
(USDA-FSIS/MLG) 8.09 [2] for beef hot dogs, deli turkey, raw chicken , sealed concrete,
43
plastic and stainless steel and AOAC 993.12 [3] for queso fresco, vanilla ice cream, 4% milk
44
fat cottage cheese and for ISO 11290-1/A1 [4] for cold smoked salmon and bagged raw
45
spinach.
46