407 / 596
e)
Filter Stomacher® bags
. - Seward or equivalent
1
f)
Thermometer
. – calibrated range to include 100 ± 1°C range
2
g)
Incubators
. – capable of maintaining 37 ± 1°C
3
h)
Dry double block heater unit.
– capable of maintaining 100 ± 1°C; or
a water bath
4
capable of maintaining 100 ± 1°C
5
i)
Refrigerator
. – capable of maintaining 2-8°C, for storing the 3M MDA2
6
j)
Computer
. – compatible with the 3M Molecular Detection System (instrument)
7
8
Safety Precautions
9
10
The user should read, understand and follow all safety information in the instructions for the 3M
11
Molecular Detection System and the 3M Molecular Detection Assay 2 -
Listeria
. Retain the
12
safety instructions for future reference.
13
14
As with all test methods, the source, formulation and quality of enrichment medium can
15
influence the results.
Factors such as sampling methods, testing protocols, sample preparation,
16
handling, and laboratory technique may also influence results. 3M recommends evaluation of
17
the method including enrichment medium, in the user’s environment using a sufficient number of
18
samples with particular foods and microbial challenges to ensure that the method meets the
19
user’s criteria.
20
The 3M Molecular Detection Assay 2 -
Listeria
method may generate
Listeria monocytogenes
to
21
levels sufficient to cause stillbirths and fatalities in pregnant women and the
22
immunocompromised, if exposed.
23
24
To reduce the risks associated with exposure to chemicals and biohazards:
•
It is strongly recommended that female laboratory staff be informed of the risk to a
developing fetus resulting from infection of the mother through exposure to
Listeria
monocytogenes
•
Perform pathogen
testing in a properly equipped laboratory under the control of trained
personnel
•
Always follow standard laboratory safety practices, including wearing appropriate protective
apparel and eye protection while handling reagents and contaminated samples
•
Avoid contact with the contents of the enrichment media and reagent tubes after amplification
•
Dispose of enriched samples according to current industry standards
25
Sample Preparation
26
3M recommends the use of Demi-Fraser Broth with FAC for the enrichment of food and
27
environmental samples. Table 1 presents guidance for the enrichment of food and environmental
28
samples. It is the user’s responsibility to validate alternate sampling protocols or dilution ratios
29
to ensure this test method meets the user’s criteria.
30
31
Foods
32
a)
Allow the Demi-Fraser Broth enrichment medium (includes FAC) to equilibrate to ambient
33
laboratory temperature.
34
b)
Aseptically combine the enrichment medium and sample according to Table 1. For all meat
35
and highly particulate samples, the use of filter bags is recommended.
36