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Test Kit Information
1
2
a)
Kit Name.
—
Molecular Detection Assay 2 –
Listeria
3
b)
Catalog Number
.
—
MDA2LIS96
4
5
Test Kit Reagents -
3M MDA 2 -
Listeria
(96 tests)
6
a)
Lysis Solution (LS) tubes
. – 96 (12 strips of 8 tubes)
7
b)
Listeria Reagent tubes
. – 96 (12 strips of 8 tubes)
8
c)
Extra caps.
– 96 (12 strips of 8 caps)
9
d)
Reagent Control (RC).
– 16 (2 pouches of 8)
10
e)
Quick Start Guide
11
12
Additional Supplies and Reagents
13
a)
3M Molecular Detection System (instrument), power supply, cord, USB cable and
14
software
15
b)
3M Molecular Detection Speed Loader Tray
16
c)
3M Molecular Detection Chill Block insert
17
d)
3M Molecular Detection Heat Block Insert
18
e)
3M Molecular Detection Cap/Decap Tool [Reagent]
19
f)
3M Molecular Detection Cap/Decap Tool [Lysis]
20
g)
Empty lysis tube rack
21
h)
Empty reagent tube rack
22
i)
Dey-Engley (D/E) Neutralizing Broth
23
j)
Demi Fraser Broth (with the addition of Ferric Ammonium Citrate [FAC])
24
k)
3M™ Hydrated Sponge Stick with 10 mL of D/E (3M Center St. Paul, MN)
25
l)
3M™ Molecular Detection Matrix Control Kit (3M Center St. Paul, MN)
26
27
Additional media
28
b)
Oxford Agar (OX)
29
c)
30
d)
Fraser Broth (FB)
31
e)
Sheep Blood Agar (SBA)
32
f)
PALCAM Agar
33
g)
Ottaviani Agosti Agar (OAA)
34
h)
Trypticase Soy Agar (TSA)
35
i)
Trypticase Soy Agar with 0.6% Yeast Extract (TSA/YE)
36
j)
De Man, Rogosa and Sharpe (MRS)
37
k)
Modified Oxford Agar (MOX)
38
l)
Brain Heart Infusion (BHI) broth
39
m)
Horse Blood Overlay (HBO)
40
n)
Buffered Listeria Enrichment Broth (BLEB)
41
42
Apparatus
43
a)
Pipettes
. – capable of 20µL
44
b)
Multi-channel pipette
. – capable of 20µL
45
c)
Sterile pipette tips.
– capable of 20µL
46
d)
Stomacher®
. – Seward or equivalent
47