AOAC-RI ERP Book MICRO.pdf

Table 1. Enrichment Protocols Using Demi-Fraser Broth Enrichmentat 37 ± 1 °C

Specific Instructions for Validated Methods

AOAC® Performance Tested Method

# 081501

In AOAC PTM studies, the 3M Molecular Detection Assay 2 –

Listeria monocytogenes

was found to be an effective method for

the detection of

Listeria monocytogenes.

The matrices tested in the study are shown in Table 3. The limit of detection of the 3M

Molecular Detection Assay 2 –

Listeria monocytogenes

method is 1-5 colony forming units per validated test portion size (in

Table 3).

Table 1.

Enrichment protocols using Demi-Fraser Broth at 37 ± 1 °C

according to AOAC Performance Tested

Certificate #081501.

Sample Matrix

Sample Size

Enrichment

Broth Volume

(mL)

Enrichment

Time (hr)

Beef hot dogs, Queso

Fresco, Vanilla Ice

Cream, 4% Milk Fat

Cottage Cheese, 3%

chocolate whole milk,

romaine lettuce, bagged

raw spinach, cold

smoked salmon

25 g

225

24-30

Formatted:

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