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AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives
1
following the reference method.Detailed results of the POD analyses are presented in Tables 10
2
and 14.
3
4
Beef Hot Dog (25 g)
5
6
For the low inoculation level of the MDA2 -
Listeria monocytogenes
assay, there were 4
7
presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference
8
method confirmation procedure, resulting in 1 false negative. There were 6 observed positives
9
for the reference method. For the high inoculation level, there were 5 presumptive positives and
10
5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
11
procedure. There were 5 confirmed positives following the reference method. Detailed results of
12
the POD analyses are presented in Tables 10 and 14.
13
14
Stainless Steel (enriched in 225 mL)
15
16
Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level
17
of the MDA2 -
Listeria monocytogenes
assay, there were 5 presumptive positives and 5
18
confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
19
procedure. There were 8 observed positives for the reference method. For the high inoculation
20
level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS
21
MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives
22
following the reference method.Detailed results of the POD analyses are presented in Tables 11
23
and 15.
24
25
Sealed Concrete (enriched in 100 mL)
26
27
24-hour primary enrichment
- For the low inoculation level of the MDA2 –
28
Listeriamonocytogenes
assay, there were 15 presumptive positives and 15 confirmed positives
29
following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 11
30
observed positives for the reference method. For the high inoculation level, there were 5
31
presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference
32
method confirmation procedure. There were 5 confirmed positives following the reference
33
method.Detailed results of the POD analyses are presented in Tables 11 and 15.
34
35
26-hour primary enrichment
- For the low inoculation level of the MDA2 –
36
Listeriamonocytogenes
assay, there were 16 presumptive positives and 15 confirmed positives
37
following the USDA/FSIS MLG 8.09 reference method confirmation procedure, resulting in 1
38
false positive. There were 11 observed positives for the reference method. For the high
39
inoculation level, there were 5 presumptive positives and 5 confirmed positives following the
40
USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed
41
positives following the reference method.Detailed results of the POD analyses are presented in
42
Tables 11 and 15.
43
44
Plastic (enriched in 10 mL)
45
46