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Challenges

ELISA kits oftentimes are used by technicians who are not experienced 

at LC/MS/MS.

Allergens are present in many matrices.

Method development to determine allergen proteins is time 

consuming.

Most food testing labs are used to using LC/MS/MS for small molecule 

contaminant testing.

Sample preparation for allergens (protein digestion) is significantly 

different from current sample prep methods (LLE, QuEChERS, SPE, etc)