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Existing LC/MS/MS Methods
•
Revision of the sample
preparation utilizing
automated online SPE
Extract proteins from
food sample (1 hour in
buffer)
•
Total sample preparation
time down to 4.5 hours
•
Results achieved through
modification:
Centrifuge and collect
extract (30 min)
Reduce, alkylate, and
di
t ith t
i (
2 5
•
Prep‐time reduced by 4x
•
Sensitivity improved by 2‐4x
•
LC‐MS/MS run time
ges w ryps n
.
hours
)
Filter digested extract
(15 min)
reduced by nearly 2x
•
Addition of more allergens
(milk, egg, peanut, treenuts,
l t
t d)
Perform automated
online SPE clean-up (
0
hours hands-on time
)
g u en, sesame, mus ar
LC-MS/MS analysis