66-67 / 427
Fitness for Purpose
XIC of +MRM (71 pairs): Exp 1, 929.986/1116.601 Da ID: [e]ELINSWVESQTNGIIR from Sample 1 (E100 mrm epi 4 allergen 161208 1) of E100 mrm e...
Max. 7100.0 cps.
1.5e4
2.0e4
2.5e4
2.8e4
nsity, cps
Egg peptides
•
Benefits of LC‐MS/MS:
–
Improved throughput
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 11.0 12.0 13.0 14.0 15.0 16.0
Time, min
0.0
5000.0
1.0e4
Inte
12.69
XIC of +MRM (71 pairs): Exp 1, 688.830/833.400 Da ID: [p]DLAFPGSGEQVEK from Sample 1 (P100 mrm epi 4 allergen 161208 1) of P100 mrm epi 4 ...
Max. 9900.0 cps.
2 0e5
2.5e5
3.0e5
ps
Peanut peptides
through multiplexing
–
Multiple allergens in
one injection
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 11.0 12.0 13.0 14.0 15.0 16.0
Time, min
0.0
5.0e4
1.0e5
1.5e5
.
Intensity, c
9.67
7.27
XIC of +MRM (71 pairs): Exp 1, 684.350/1183.570 Da ID: [m]ALNEINQFYQK from Sample 1 (M100 mrm epi 4 allergen 161208 1) of M100 mrm epi 4 all...
Max. 7.7e4 cps.
8.0e5
8.8e5
Milk peptides
–
Multiple fragments for
each allergen detected
More confidence for
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 11.0 12.0 13.0 14.0 15.0 16.0
Time, min
0.0
1.0e5
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
7.0e5
Intensity, cps
10.08
–
fewer false positive or
false negative results
Multi‐allergen analysis